Advance of <i>in vitro</i> oral static biofilm model.

Huabing Zhao; Tingting Shi; Fengzhu LI; Rui Wang; Fuping LU; Zhengmei Huang

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Advance of in vitro oral static biofilm model.

Author: Huabing ZHAO ¹ ; Tingting SHI ¹ ; Fengzhu LI ¹ ; Rui WANG ¹ ; Fuping LU ¹ ; Zhengmei HUANG ²
Author Information

1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China.
2. Tianke & Benzhen Human microecology Research Institute, Tianke & Benzhen (Tianjin) Biotechnology Company Limited, Tianjin 300457, China.
Publication Type:Journal Article
Keywords: Amsterdam active attachment model; Calgary biofilm device; biofilm; microcosms models; multispecies biofilm models; oral; static model
MeSH: Biofilms; Reproducibility of Results
From: Chinese Journal of Biotechnology 2022;38(9):3267-3278
CountryChina
Language:Chinese
Abstract: Oral static biofilm model is an important tool for in vitro simulation of oral microecological environment, which has become an important method for studying the pathogenesis of various oral diseases and testing the efficacy of various drugs, oral care products and foods due to its low cost, high throughput, good reliability and easy operation. The establishment of oral static biofilm models allows the selection of different devices, inoculum sources, media, substrates and culture conditions according to the purpose of the study, and the evaluation of biofilm growth by various methods such as measuring biomass, metabolic activity, community structure and performing visualization analysis. This paper summarizes the methodological elements reported in recent years for the establishment and evaluation of oral static biofilm models, and analyzes and discusses the applicability of various methods in the hope of contributing to the research and production practice in related fields.