Overexpression of ATF3 inhibits the differentiation of goat intramuscular preadipocytes.
- Author:
Chongyang WANG
1
;
Cheng LUO
1
;
Hao ZHANG
1
;
Xin LI
1
;
Yanyan LI
1
;
Yan XIONG
1
;
Youli WANG
1
;
Yaqiu LIN
1
Author Information
- Publication Type:Journal Article
- Keywords: ATF3; goat; induced differentiation; intramuscular preadipocytes; overexpression
- MeSH: 3T3-L1 Cells; Activating Transcription Factor 3/pharmacology*; Adipocytes; Adipogenesis/genetics*; Animals; CCAAT-Enhancer-Binding Protein-alpha/pharmacology*; Cell Differentiation; Goats; Mice; PPAR gamma/metabolism*
- From: Chinese Journal of Biotechnology 2022;38(8):2939-2947
- CountryChina
- Language:Chinese
- Abstract: The aim of this study was to investigate the effect of activating transcription factor 3 (ATF3) on the differentiation of intramuscular preadipocytes in goat, and to elucidate its possible action pathway at the molecular level. In this study, the recombinant plasmid of goat pEGFP-N1-ATF3 was constructed, and the intramuscular preadipocytes were transfected with liposomes. The relative expression levels of adipocyte differentiation marker genes were detected by quantitative real-time PCR (qRT-PCR). After transfection of goat intramuscular preadipocytes with the goat pEGFP-N1-ATF3 overexpression vector, it was found that the accumulation of lipid droplets was inhibited, and the adipocyte differentiation markers PPARγ, C/EBPα and SREBP1 were extremely significantly down-regulated (P < 0.01), while C/EBPβ and AP2 were significantly down-regulated (P < 0.05). The ATF3 binding sites were predicted to exist in the promoter regions of PPARγ, C/EBPα and AP2 by the ALGGEN PROMO program. The overexpression of goat ATF3 inhibits the accumulation of lipid droplets in intramuscular preadipocytes, and this effect may be achieved by down-regulating PPARγ, C/EBPα and AP2. These results may facilitate elucidation of the regulatory mechanism of ATF3 in regulating the differentiation of goat intramuscular preadipocytes.
