Identification of host proteins interacting with African swine fever virus inner envelope protein p17.

Guifang Zhong; Tingjuan Deng; Kang XU; Wenbi NI; Pei Wang; Boli HU; Jiyong Zhou

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Identification of host proteins interacting with African swine fever virus inner envelope protein p17.

Author: Guifang ZHONG ¹ ; Tingjuan DENG ¹ ; Kang XU ¹ ; Wenbi NI ¹ ; Pei WANG ¹ ; Boli HU ¹ ; Jiyong ZHOU ¹
Author Information

1. MOA Key Laboratory of Animal Virology/Center of Veterinary Science, Zhejiang University, Hangzhou 310058, Zhejiang, China.
Publication Type:Journal Article
Keywords: African swine fever virus; immunoprecipitation; interacting protein; mass spectrometry; p17
MeSH: African Swine Fever; African Swine Fever Virus/metabolism*; Animals; Open Reading Frames; Swine; Viral Proteins/metabolism*
From: Chinese Journal of Biotechnology 2022;38(8):2883-2890
CountryChina
Language:Chinese
Abstract: African swine fever virus (ASFV) infection leads to a mortality rate of up to 100%, causing devastating disasters to the pig industry. Understanding the ASFV infection and replication is therefore of great importance. ASFV has more than 150 open reading frames, among which the inner coat protein p17 encoded by the D117L gene is involved in the formation of the icosahedral structure of the virus. However, little is known about the mechanism how p17 regulates host cell function. In this study, the potential host proteins interacting with ASFV p17 were screened by immunoprecipitation technique combined with protein profiling analysis. The interactions of p17 with mitochondrial membrane protein TOMM70 and heat shock protein HSPA8 were confirmed by co-immunoprecipitation technique and laser confocal experiments. This study provides important information for further exploring the function of p17 during ASFV infection.