Differential mRNA Expressions in HCMV infected HUVECs.

Chang Ning Lyu; Ji Chen LI; Qi AN; Min Zhang; Yan Jun Zong; Zhong Fa Yang; Xiang Yu Zou; Fu Jun Peng; Qin Wang; Zhi Jun Liu

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Differential mRNA Expressions in HCMV infected HUVECs.

Author: Chang Ning LYU ¹ ; Ji Chen LI ² ; Qi AN ¹ ; Min ZHANG ¹ ; Yan Jun ZONG ² ; Zhong Fa YANG ² ; Xiang Yu ZOU ² ; Fu Jun PENG ² ; Qin WANG ³ ; Zhi Jun LIU ²
Author Information

1. School of Clinical Medicine, Weifang Medical University, Weifang 261053, Shandong, China.
2. School of Basic Medical Sciences, Weifang Medical University, Weifang 261053, Shandong, China.
3. National Institute for Viral Diseases Control and Prevention, China Center for Disease Control and Prevention, Beijing 102206, China.
Publication Type:Journal Article
Keywords: Atherosclerosis; Cell injury; Cytomegalovirus; Endothelial cells; Gene expressions; RNA microarrays
MeSH: Humans; Cytomegalovirus/genetics*; Human Umbilical Vein Endothelial Cells; Atherosclerosis; Protein Kinases; RNA, Messenger
From: Biomedical and Environmental Sciences 2022;35(10):888-898
CountryChina
Language:English
Abstract: OBJECTIVE:The aim was to identify the gene expressions of human cytomegalovirus (HCMV)-infected human umbilical vein endothelial cells (HUVECs) and to study its possible pathogenic mechanism on atherosclerosis using microarray technology.
METHODS:The gene expression differences in HCMV AD169 strain-infected HUVECs were studied by the microarray technology to explore the potential molecular mechanism of HCMV infection. The qPCRs were performed to verify the transcriptome results.
RESULTS:A total of 2,583 differentially expressed genes, including 407 down-regulated genes and 2,176 up-regulated genes, were detected by the systematic bioinformatics analysis. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that the significantly differentially expressed genes were mainly involved in regulating protein kinase activity, inflammatory response, ubiquitination, protein phosphorylation, cell metabolism, and exosomes, among which 12 genes had significant changes and were screened by protein-protein interaction (PPI) analysis and verified by qPCR. The experimental qPCR results were consistent with the microarray results.
CONCLUSION:The GO and KEGG analyses revealed that the regulation of protein kinase activity, inflammatory response, ubiquitination, protein phosphorylation, and cell metabolism played important roles in the process of endothelial cell infection. Furthermore, 12 genes were involved in the process of HCMV infection of endothelial cells and contributed to the current understanding of the infection and pathogenic mechanisms of atherosclerosis.