Effects of Combination of 1,25(OH) 2D 3 and TLR-4 Inhibitor on the Damage to HaCaT Cells Caused by UVB Irradiation.
- Author:
Peng CHEN
1
;
Chuan Ning ZHUANG
2
;
Jia Jing CUI
2
;
Ping Wei WANG
2
;
Dong Ge LIU
2
;
Shu Qi YAN
2
;
Li Ting ZHOU
2
;
Shu Ping REN
2
Author Information
- Publication Type:Journal Article
- Keywords: 1,25(OH)2D3; Combined effects; HaCaT cell; Toll-like receptor 4; UVB
- MeSH: Humans; HaCaT Cells; NF-kappa B; Reactive Oxygen Species; Toll-Like Receptor 4; Ultraviolet Rays/adverse effects*; Cholecalciferol/analogs & derivatives*
- From: Biomedical and Environmental Sciences 2022;35(11):1051-1062
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:Vitamin D and Toll-like receptor-4 (TLR-4) inhibition are involved in the protection of keratinocytes. The effects of combination of 1,25(OH) 2D 3 and TLR-4 inhibitor on the protection of keratinocytes against ultraviolet radiation B (UVB) irradiation remain unclear. This study was undertaken to explore the effects of combination of 1,25(OH) 2D 3 and TAK-242 (TLR-4 inhibitor) on the damage to HaCaT cells caused by UVB irradiation.
METHODS:In vitro, HaCaT cells were treated with 1,25(OH) 2D 3 or/and TAK-242 prior to UVB irradiation at the intensity of 20 mJ/cm 2, then the production of reactive oxygen species (ROS), cell migration, apoptosis of cells, and the expression of oxidative stress, endoplasmic reticulum stress, and apoptosis related proteins were determined.
RESULTS:Compared with the HaCaT cells treated with 1,25(OH) 2D 3 or TAK-242, the cells treated with both 1,25(OH) 2D 3 and TAK-242 showed, 1) significantly lower production of ROS ( P < 0.05); 2) significantly less apoptosis of HaCaT cells ( P < 0.05); 3) significantly lower expression of NF- κB, Caspase-8, Cyto-C, Caspase-3 ( P < 0.05).
CONCLUSION:The combination of 1,25(OH) 2D 3 and TAK-242 could produce a better protection for HaCaT cells via inhibiting the oxidative stress, endoplasmic reticulum stress and apoptosis than 1,25(OH) 2D 3 or TAK-242 alone.
