Construction of MicroRNA-Target Interaction Networks Based on MicroRNA Expression Profiles of HRV16-infected H1-HeLa Cells.
- Author:
Qin Qin SONG
1
;
Yan Hai WANG
1
;
Xin Ling WANG
1
;
Bing Tian SHI
1
;
Rui Fang WANG
1
;
Juan SONG
1
;
Wen Jun WANG
1
;
Dong XIA
1
;
Zhi Qiang XIA
1
;
Qiang WEI
2
;
Jun HAN
1
Author Information
- Publication Type:Letter
- Keywords: HRV16; MicroRNAs; Targets
- MeSH: CELF Proteins/metabolism*; DNA-Binding Proteins/genetics*; Gene Expression Profiling; Gene Expression Regulation; HeLa Cells; High-Throughput Nucleotide Sequencing; Humans; MicroRNAs/metabolism*; Nerve Tissue Proteins/genetics*; Protein Tyrosine Phosphatases, Non-Receptor; Repressor Proteins/metabolism*; Sequence Analysis, RNA; Transcription Factors/metabolism*
- From: Biomedical and Environmental Sciences 2022;35(9):854-860
- CountryChina
- Language:English
- Abstract: In the present study we investigated the changes in miRNA levels inhuman rhinovirus 16 (HRV16)-infected cells. A small RNA deep sequencing experiment was performed through next-generation sequencing. In total, 53 differentially expressed miRNAs were confirmed by RT-qPCR, including 37 known miRNAs and 16 novel miRNAs. Interaction networks between differentially expressed miRNAs and their targets were established by mirDIP and Navigator. The prediction results showed that QKI, NFAT5, BNC2, CELF2, LCOR, MBNL2, MTMR3, NFIB, PPARGC1A, RSBN1, TRPS1, WDR26, and ZNF148, which are associated with cellular differentiation and transcriptional regulation, were recognized by 12, 11, or 9 miRNAs. Many correlations were observed between transcriptional or post-transcriptional regulation of an miRNA and the expression levels of its target genes in HRV16-infected H1-HeLa cells.
