H-NS Represses Biofilm Formation and c-di-GMP Synthesis in <i>Vibrio parahaemolyticus</i>.

Xing Fan XUE; Miao Miao ZHNAG; Jun Fang SUN; Xue LI; Qi Min WU; Zhe YIN; Wen Hui YANG; Bin NI; Ling Fei HU; Dong Sheng ZHOU; Ren Fei LU; Yi Quan ZHANG

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H-NS Represses Biofilm Formation and c-di-GMP Synthesis in Vibrio parahaemolyticus.

Author: Xing Fan XUE ¹ ; Miao Miao ZHNAG ¹ ; Jun Fang SUN ² ; Xue LI ² ; Qi Min WU ² ; Zhe YIN ³ ; Wen Hui YANG ³ ; Bin NI ¹ ; Ling Fei HU ³ ; Dong Sheng ZHOU ³ ; Ren Fei LU ² ; Yi Quan ZHANG ²
Author Information

1. School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
2. Department of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong 226006, Jiangsu, China.
3. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Publication Type:Journal Article
Keywords: Biofilm; C-di-GMP; H-NS; Vibrio parahaemolyticus
MeSH: Bacterial Proteins/metabolism*; Biofilms; Cyclic GMP/analogs & derivatives*; Gene Expression Regulation, Bacterial; Gentian Violet; Histones/metabolism*; Vibrio parahaemolyticus/genetics*
From: Biomedical and Environmental Sciences 2022;35(9):821-829
CountryChina
Language:English
Abstract: OBJECTIVE:This study aimed to investigate the regulation of histone-like nucleoid structuring protein (H-NS) on biofilm formation and cyclic diguanylate (c-di-GMP) synthesis in Vibrio parahaemolyticus RIMD2210633.
METHODS:Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining, quantification of c-di-GMP, quantitative real-time polymerase chain reaction, LacZ fusion, and electrophoretic-mobility shift assay.
RESULTS:The deletion of hns enhanced the biofilm formation and intracellular c-di-GMP levels in V. parahaemolyticus RIMD2210633. H-NS can bind the upstream promoter-proximal DNA regions of scrA, scrG, VP0117, VPA0198, VPA1176, VP0699, and VP2979 to repress their transcription. These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism.
CONCLUSION:One of the mechanisms by which H-NS represses the biofilm formation by V. parahaemolyticus RIMD2210633 may be via repression of the production of intracellular c-di-GMP.