The Viral Load of Epstein-Barr Virus in Blood of Children after Hematopoietic Stem Cell Transplantation.

Wen Jun Wang; Shun Qiao Feng; Feng HE; Hai Jun DU; Miao Feng; Rui Fang Wang; Guo Yong Mei; Mi Liu; Rong Liu; Hai Lan Yao; Jun Han

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The Viral Load of Epstein-Barr Virus in Blood of Children after Hematopoietic Stem Cell Transplantation.

Author: Wen Jun WANG ¹ ; Shun Qiao FENG ² ; Feng HE ³ ; Hai Jun DU ¹ ; Miao FENG ³ ; Rui Fang WANG ¹ ; Guo Yong MEI ¹ ; Mi LIU ¹ ; Rong LIU ² ; Hai Lan YAO ³ ; Jun HAN ¹
Author Information

1. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
2. Department of Hematology, Children's Hospital of Capital Institute of Pediatrics, Beijing 100020, China.
3. Department of Biochemistry & Immunology, Capital Institute of Pediatrics, Beijing 100020, China.
Publication Type:Journal Article
Keywords: Chip digital PCR; Epstein-Barr virus; Hematopoietic stem cell transplantation; Quantitative PCR
MeSH: Child; DNA, Viral/analysis*; Epstein-Barr Virus Infections/epidemiology*; Hematopoietic Stem Cell Transplantation/adverse effects*; Herpesvirus 4, Human/genetics*; Humans; Real-Time Polymerase Chain Reaction; Viral Load
From: Biomedical and Environmental Sciences 2022;35(9):804-810
CountryChina
Language:English
Abstract: OBJECTIVE:To detect the Epstein-Barr virus (EBV) viral load of children after hematopoietic stem cell transplantation (HSCT) using chip digital PCR (cdPCR).
METHODS:The sensitivity of cdPCR was determined using EBV plasmids and the EBV B95-8 strain. The specificity of EBV cdPCR was evaluated using the EBV B95-8 strain and other herpesviruses (herpes simplex virus 1, herpes simplex virus 2, varicella zoster virus, human cytomegalovirus, human herpesvirus 6, and human herpesvirus 7). From May 2019 to September 2020, 64 serum samples of children following HSCT were collected. EBV infection and the viral load of serum samples were detected by cdPCR. The epidemiological characteristics of EBV infections were analyzed in HSCT patients.
RESULTS:The limit of detection of EBV cdPCR was 110 copies/mL, and the limit of detection of EBV quantitative PCR was 327 copies/mL for the pUC57-BALF5 plasmid. The result of EBV cdPCR was up to 121 copies/mL in the EBV B95-8 strain, and both were more sensitive than that of quantitative PCR. Using cdPCR, the incidence of EBV infection was 18.75% in 64 children after HSCT. The minimum EBV viral load was 140 copies/mL, and the maximum viral load was 3,209 copies/mL using cdPCR. The average hospital stay of children with EBV infection (184 ± 91 days) was longer than that of children without EBV infection (125 ± 79 days), P = 0.026.
CONCLUSION:EBV cdPCR had good sensitivity and specificity. The incidence of EBV infection was 18.75% in 64 children after HSCT from May 2019 to September 2020. EBV cdPCR could therefore be a novel method to detect EBV viral load in children after HSCT.