Analysis of polymorphic regions of Plasmodium vivax Duffy binding protein of Korean isolates.
10.3347/kjp.2001.39.2.143
- Author:
Weon Gyu KHO
1
;
Joon Yong CHUNG
;
Eun Jeong SIM
;
Dong Wook KIM
;
Woo Chul CHUNG
Author Information
1. Department of Parasitology, Institute of Malariology, Inje University College of Medicine, Pusan 614-735, Korea. wgkho@ijnc.inje.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH:
Amino Acid Sequence;
Animals;
*Antigens, Protozoan;
Base Sequence;
Carrier Proteins/*analysis/chemistry/*genetics;
DNA, Protozoan/genetics;
Genotype;
Human;
Korea;
Malaria, Vivax/parasitology;
Molecular Sequence Data;
Plasmodium vivax/*genetics/isolation & purification;
Polymerase Chain Reaction;
*Polymorphism (Genetics);
*Protozoan Proteins;
Receptors, Cell Surface/*analysis/chemistry/*genetics;
Support, Non-U.S. Gov't
- From:The Korean Journal of Parasitology
2001;39(2):143-150
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present study was designed to investigate polymorphism in Duffy binding protein (DBP) gene of Plasmodium vivax isolates of Korea. Thirty samples were obtained from P. vivax patients in Yonchon-gun, Kyonggi-do in 1998. The PCR products of the samples were subjected to sequencing and hybridization analyses of the regions II and IV of P. vivax DBP gene. Two genotypes, SK-1 and SK-2, were identified on the basis of amino acid substitution and deletion. The genotype of 10 isolates was SK-1 and that of 20 isolates was SK-2. Most of the predicted amino acids in the region II of DBP gene were conserved between the Korean isolates and Belem strain except for 4-5 amino acid substitutions. In the region IV of DBP, a 6-bp insert that was shown in the Sal-1 allele type was found in SK-1, and a 27-bp insert that was shown in the Papua New Guinea allele type was found in SK-2. In conclusion, the present findings suggest that two genotypes of P. vivax coexist in the endemic area of Korea.
