Experimental infection of Anopheles sinensis with Korean isolates of Plasmodium vivax.
10.3347/kjp.2001.39.2.177
- Author:
Hyeong Woo LEE
1
;
Shin Hyeong CHO
;
E Hyun SHIN
;
Jong Soo LEE
;
Joon Sang LEE
;
Jong Yil CHAI
;
Soon Hyung LEE
;
Tong Soo KIM
Author Information
1. Department of Medical Zoology, National Institute of Health, Seoul, 122-701, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Anopheles/*parasitology;
Enzyme-Linked Immunosorbent Assay;
Human;
Korea;
Plasmodium vivax/isolation & purification/*pathogenicity;
Support, Non-U.S. Gov't
- From:The Korean Journal of Parasitology
2001;39(2):177-183
- CountryRepublic of Korea
- Language:English
-
Abstract:
The objectives of the present study were to (1) determine the susceptibility of Anopheles sinensis to Korean isolates of Plasmodium vivax, (2) establish a method to collect large quantities of P. vivax sporozoites for use as antigen in seroepidemiological studies, and (3) investigate the characteristics of Korean isolates of P. vivax sporozoites. Females of Anopheles sinensis were collected at non-epidemic area, Seokwha-ri, Cheongwon-gun and Chungcheongbuk-do using tent-trap methods coupled with dry ice. The females were artificially infected with gametocytes of P. vivax using blood obtained from P. vivax malaria patients. Individual mosquitoes were infected using either a parafilm-covered glass feeding apparatus or were allowed to feed on naturally infected volunteers. Mosquitoes were sacrificed between 16 and 18 days post-feeding and an enzyme-linked immunosorbent assay (ELISA) was used to detect sporozoites. Four (33.4%) of 12 mosquitoes, which were fed on naturally infected volunteers directly, were positive for sporozoites. In cases, the mosquitoes allowed to feed on whole blood which were extract from three different patients with heparin treated vacuutainers using a parafilm-covered glass apparatus. Two of 55 (3.6%) were positive which blood sample was maintained at room temperature for 8 hours, 1 of 68 (1.5%) was positive which blood was maintained at 4 degrees C for 24 hours and 1 of 47 (2.3%) was positive at 4 degrees C for 48 hours. The mean number of sporozoites was estimated about 818 (n = 8; range of 648-1,056) based on optical density values of ELISA.
