Effects of Jinmaitong on Expression of Bax, Bcl-2 and Caspase-3 in Hippocampal Neurons Cultured with High Glucose
10.3969/j.issn.1006-9771.2012.04.003
- VernacularTitle:中药筋脉通对高糖培养海马神经元细胞Bax、Bcl-2 和Caspase-3蛋白表达的影响
- Author:
Leilei GUO
;
Hong ZHANG
;
Guoqing TIAN
;
Xiaochun LIANG
- Publication Type:Journal Article
- Keywords:
diabetes mellitus, cognitive dysfunction, Jinmaitong, hippocampal neurons, Bax, Bcl-2, Caspase-3
- From:
Chinese Journal of Rehabilitation Theory and Practice
2012;18(4):324-328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Jinmaitong (JMT) on expression of Bax, Bcl-2 and Caspase-3 in hippocampal neurons culturedwith high glucose. Methods Hippocampal neurons were primarily cultured and purified from the hippocampus of new-born SpragueDawley rats. Neuron-specific enolase immunocytochemical method was adopted for the identification of the neurons. They were divided intonormal control group, high glucose group, high-dose JMT (JH) group, middle-dose JMT (JM) group, low-dose JMT (JL) group and a positivecontrol group. 72 hours later, Western blotting was adopted to detect the expression of Bax, Bcl-2 and Caspase-3. Results Comparedwith the normal control group, the expression of Bax and Caspase-3 in the high glucose group significantly increased (P<0.01), and the expressionof Bcl-2 significantly decreased (P<0.05). Compared with the high glucose group, the expression of Caspase-3 and Bax in the positivecontrol group and JH, JM, and JL groups significantly decreased (P<0.01), and the expression of Bcl-2 significantly increased (P<0.05).Compared with the positive control group, the expression of Caspase-3 and Bax significantly decreased in JH, JM, and JL groups (P<0.05),and the expression of Bcl-2 significantly increased in JH and JM groups (P<0.05). Conclusion JMT may reduce apoptosis by inhibiting theexpression of Bax and Caspase-3 and promoting the expression of Bcl-2.
