Stem cells from human exfoliated deciduous teeth attenuate trigeminal neuralgia in rats by inhibiting endoplasmic reticulum stress
10.3344/kjp.2022.35.4.383
- Author:
Zhijie YANG
1
;
Chun WANG
;
Xia ZHANG
;
Jing LI
;
Ziqi ZHANG
;
Zhao TAN
;
Junyi WANG
;
Junyang ZHANG
;
Xiaofeng BAI
Author Information
1. Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China Medical University, Shenyang, China
- Publication Type:Experimental Research Article
- From:The Korean Journal of Pain
2022;35(4):383-390
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:The treatment of trigeminal neuralgia remains a challenging issue.Stem cells from human exfoliated deciduous teeth (SHED) provide optimized therapy for chronic pain. This study aimed to investigate the mechanisms underlying the attenuation of trigeminal neuralgia by SHED.
Methods:Trigeminal neuralgia was induced by chronic constriction injury of the infraorbital nerve. The mechanical threshold was assessed after model establishment and local SHED transplantation. Endoplasmic reticulum (ER) morphology and Caspase12 expression in trigeminal ganglion (TG) was evaluated as well. BiP expression was observed in PC12 cells induced by tunicamycin.
Results:The local transplantation of SHED could relieve trigeminal neuralgia in rats.Further, transmission electron microscopy revealed swelling of the ER in rats with trigeminal neuralgia. Moreover, SHED inhibited the tunicamycin-induced up-regulated expression of BiP mRNA and protein in vitro. Additionally, SHED decreased the up-regulated expression of Caspase12 mRNA and protein in the TG of rats caused by trigeminal neuralgia after chronic constriction injury of the infraorbital nerve mode.
Conclusions:This findings demonstrated that SHED could alleviate pain by relieving ER stress which provide potential basic evidence for clinical pain treatment.
