Quantitative Analysis Of Random Chromosomal Aberrations In PHA-Stimulated Blood and Bone Marrow.
- Author:
Hye Ryoung YOON
1
;
Sun Hee KIM
Author Information
1. Seoul Clinical Laboratories(SCL), Seoul Medical Science Institute.
- Publication Type:In Vitro ; Original Article
- Keywords:
Random chromosomal aberrations;
Quality control;
PHA-stimulated blood;
Bone marrow
- MeSH:
Aneuploidy;
Artifacts;
Bone Marrow*;
Chromosome Aberrations*;
Chromosomes, Human, 4-5;
Clone Cells;
Metaphase;
Quality Control
- From:Korean Journal of Clinical Pathology
2000;20(1):113-
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Chromosomal aberration observed only in a few metaphases may cause the cytogeneticists to have difficulties in making a decision whether it is due to in vivo mosaicism/multiple clones or due to in vitro artifact. This is especially important when the chromosome of concern has been associated with a classical chromosome syndrome, malignancy or its evolution. Therefore, we aimed to establish a range for random chromosomal aberrations among cells from PHA-stimulated blood(PB) and bone marrow(BM) cultures. METHODS: Among the cells from 449 PB and 472 BM specimens referred for chromosome studies from 1997 to 1998, we analyzed the frequency of random aneuploidy, structural abnormalities, and breaks/gaps. RESULTS: The number of cells analyzed was 5,904/4,488(1997/1998) in PB and 4,211/4,124(1997/1998) in BM. The frequency of metaphases with random chromosomal aberrations of BM(32.10%) was much higher than that of PB(5.90%). The most frequent aberration was chromsome loss. Autosome losses were inversely correlated with autosome size(correlation coefficient = -0.83 and -0.72, p<0.01), smaller chromosomes being lost more frequently while autosome breaks/gaps were correlated with autosome size(correlation coefficient = 0.69 and 0.85, p<0.01), in PB and BM. Comparing the data from 1998 to the data from 1997, the frequency of chromosome losses(<0.5% in PB, <2.25% in BM), gains(<0.1% in PB and BM), breaks/gaps(<0.1% in PB, <0.25% in BM), and structural aberrations(
