Humulus japonicus attenuates LPS-and scopolamine-induced cognitive impairment in mice
10.1186/s42826-022-00134-3
- Author:
Jun GO
1
;
Hye-Yeon PARK
;
Da Woon LEE
;
So-Young MAENG
;
In-Bok LEE
;
Yun Jeong SEO
;
Jin-Pyo AN
;
Won Keun OH
;
Chul-Ho LEE
;
Kyoung-Shim KIM
Author Information
1. Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, Gwahak-ro 125, Yuseong-gu, Daejeon 34141, Republic of Korea.
- Publication Type:RESEARCH
- From:Laboratory Animal Research
2022;38(3):159-168
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Neuroinflammation plays an important role in cognitive decline and memory impairment in neurodegenerative disorders. Previously, we demonstrated that Humulus japonicus (HJ) has anti-inflammatory effects in rodent models of Alzheimer’s disease and Parkinson’s disease. The present study aimed to examine the protective potential of HJ extracts against lipopolysaccharide (LPS)-induced cognitive impairment and scopolamine-induced amnesia in mouse models. Cognitive improvement of mice was investigated by novel object recognition test. For analyzing effects on neuroinflammation, immunohistochemistry and quantitative real-time polymerase chain reaction (qRTPCR) assays were performed.
Results:We found that the oral administration of HJ significantly improved cognitive dysfunction induced by LPS in a novel object recognition test. The LPS-induced activation of microglia was notably decreased by HJ treatment in the cortex and hippocampus. HJ administration with LPS also significantly increased the mRNA expression of interleukin (IL)-10 and decreased the mRNA expression of IL-12 in the parietal cortex of mice. The increased expression of LPS-induced complement C1q B chain (C1bq) and triggering receptor expressed on myeloid cells 2 (Trem2) genes was significantly suppressed by HJ treatment. In addition, HJ administration significantly improved novel object recognition in a scopolamine-induced amnesia mouse model.
Conclusions:These findings revealed that HJ has a beneficial effect on cognitive impairment and neuroinflammation induced by systemic inflammation and on amnesia induced by scopolamine in mice.
