Increased Viability by Over-Expression of Bcl-XL in a Human Neural Progenitor Cell Line.
- Author:
Young Hwan AHN
1
;
Yong Sam SHIN
;
Soo Han YOON
;
Ki Hong CHO
;
Kyung Gi CHO
;
Seung Up KIM
Author Information
1. Department of Neurosurgery, Ajou University School of Medicine, Suwon, Korea. yhahn@ajou.ac.kr
- Publication Type:Case Report
- Keywords:
Bcl-XL;
Human neural progenitor cell;
Overexpression;
Transfection
- MeSH:
Antibodies;
Blotting, Western;
Cell Death;
Humans*;
Microscopy, Fluorescence;
Nestin;
Stem Cells*;
Transfection;
Transplants
- From:Journal of Korean Neurosurgical Society
2004;35(4):415-421
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: This study is aimed whether bcl-xl could protect C4 cells from the cell death induced by serum deprivation. METHODS: The transient transfection of the bcl-xl gene was made with a LipofectAMINE reagent. An immunohistocytochemical assay and Western-blotting were performed to examine the bcl-xl transfection into the C4 cells. In order to analyze the effect of the bcl-xl transfection, the number of cells on the well plate were serially counted each day, for 5 days, from the 2nd to the 6th day after transfection. The number of GFP-positive cells in the defined fields, following serum deprivation, was counted using fluorescence microscopy, and the total number of viable cells, including transfected cells, were also assessed. RESULTS: Immunocytochemical staining showed positive cells in 52% of nestin staining, 60% of GFAP and 20% of MAP-2. The number of cells decreased after transfection using the LipofectAMINE in the serum free medium (p<0.001). Western blotting using an anti-human bcl-xl antibodies showed that bcl-xl was expressed in both the non-transfected and bcl-xl transfected C4 cells. Cell death in the C4 cells, and the number of cells, were serially monitored each day for 5 days. In the bcl-xl transfected cells, the cell death induced by serum deprivation was significantly inhibited and delayed compared to that in the control cells (p<0.001). CONCLUSION: It is suggested that the bcl-xl transfected human neural progenitor cells might improve the survival of the grafted cells, and may be an alternative source of cells for neural transplantation in degenerative diseases.
