A study on the effect of microgroove-fibronectin complex titanium plate on the expression of various cell behavior-related genes in human gingival fibroblasts
10.14368/jdras.2022.38.3.150
- Author:
Yu Jeong HWANG
1
;
Won Joong LEE
;
Richard LEESUNGBOK
;
Suk Won LEE
Author Information
1. Department of Biomaterials & Prosthodontics, Kyung Hee University College of Dentistry, Kyung Hee Unoversity Hospital at Gangdong, Seoul, Republic of Korea
- Publication Type:Original Article
- From:Journal of Dental Rehabilitation and Applied Science
2022;38(3):150-161
- CountryRepublic of Korea
- Language:English
-
Abstract:
Purpose:To determine the effects of the microgroove-fibronectin complex surface on the expression of various genes related to cel-lular activity in human gingival fibroblasts.
Materials and Methods:Smooth titanium specimens (NE0), acid-treated titanium speci-mens (E0), microgroove and acid-treated titanium specimens (E60/10), fibronectin-fixed smooth titanium specimens (NE0FN), acidtreated and fibronectin-immobilized titanium specimens (E0FN), and microgroove and acid-treated titanium specimens immobilized with fibronectin (E60/10FN) were prepared. Real-time polymerase chain reaction experiments were conducted on 44 genes related to cell behavior of human gingival fibroblasts.
Results:Adhesion and proliferation of human gingival fibroblast on microgroovefibronectin complex titanium were activated through four types of signaling pathway. Integrin α5, Integrin β1, Integrin β3, Talin-2, which belong to the focal adhesion pathway, AKT1, AKT2, NF-κB, which belong to the PI3K-AKT signaling pathway, MEK2, ERK1, ERK2, which belong to the MAPK signaling pathway, and Cyclin D1, CDK4, CDK6 genes belonging to the cell cycle signaling pathwaywere upregulated on the microgroove-fibronectin complex titanium surface (E60/10FN).
Conclusion:The microgroove-fibronectin complex titanium surface can up-regulate various genes involved in cell behavior.
