Comparative Study of Autophagy in Oxaliplatin-Sensitive and Resistant SNU-C5 Colon Cancer Cells
10.4062/biomolther.2022.028
- Author:
Sun-Jin BOO
1
;
Mei Jing PIAO
;
Kyoung Ah KANG
;
Ao Xuan ZHEN
;
Pincha Devage Sameera Madushan FERNANDO
;
Herath Mudiyanselage Udari Lakmini HERATH
;
Seung Joo LEE
;
Seung Eun SONG
;
Jin Won HYUN
Author Information
1. Department of Internal Medicine, Jeju National University Hospital, College of Medicine, Jeju National University, Jeju 63241, Republic of Korea
- Publication Type:Original Article
- From:Biomolecules & Therapeutics
2022;30(5):447-454
- CountryRepublic of Korea
- Language:English
-
Abstract:
Few studies have evaluated the role of autophagy in the development of oxaliplatin (OXT) resistance in colon cancer cells. In this study, we compared the role of autophagy between SNU-C5 colon cancer cells and OXT-resistant SNU-C5 (SNU-C5/OXTR) cells. At the same concentration of OXT, the cytotoxicity of OXT or apoptosis was significantly reduced in SNU-C5/OXTR cells compared with that in SNU-C5 cells. Compared with SNU-C5 cells, SNU-C5/OXTR cells exhibited low levels of autophagy. The expression level of important autophagy proteins, such as autophagy-related protein 5 (Atg5), beclin-1, Atg7, microtubule-associated proteins 1A/1B light chain 3B I (LC3-I), and LC3-II, was significantly lower in SNU-C5/OXTR cells than that in SNU-C5 cells. The expression level of the autophagy-essential protein p62 was also lower in SNU-C5/OXTR cells than in SNU-C5 cells. In SNUC5/OXTR cells, the production of intracellular reactive oxygen species (ROS) was significantly higher than that in SNU-C5 cells, and treatment with the ROS scavenger N-acetylcysteine restored the reduced autophagy levels. Furthermore, the expression of antioxidant-related nuclear factor erythroid 2-related factor 2 transcription factor, heme oxygenase-1, and Cu/Zn superoxide dismutase were also significantly increased in SNU-C5/OXTR cells. These findings suggest that autophagy is significantly reduced in SNU-C5/OXTR cells compared with SNU-C5 cells, which may be related to the production of ROS in OXT-resistant cells.
