The Effects of Glucose on the Expression of MMP and TIMP in Cultured Retinal Pigment Epithelial Cells.
10.3341/kjo.2004.18.2.132
- Author:
Dongeun LEE
1
;
Jae Suk KIM
;
Ho Kyun CHO
;
Joo Hwa LEE
Author Information
1. 21c Eye Hospital, Inje University, College of Medicine, Seoul Paik Hospital, Korea.
- Publication Type:Original Article ; Comparative Study ; In Vitro ; Research Support, Non-U.S. Gov't
- Keywords:
glucose;
human retinal pigment epithelial cell;
MMP;
TIMP
- MeSH:
Blotting, Western;
Cell Movement;
Cell Proliferation;
Cells, Cultured;
Comparative Study;
Dose-Response Relationship, Drug;
Glucose/*pharmacology;
Humans;
In Vitro;
Matrix Metalloproteinases/genetics/*metabolism;
Pigment Epithelium of Eye/*drug effects/enzymology;
RNA, Messenger/metabolism;
Research Support, Non-U.S. Gov't;
Reverse Transcriptase Polymerase Chain Reaction;
Time Factors;
Tissue Inhibitor of Metalloproteinases/genetics/*metabolism
- From:Korean Journal of Ophthalmology
2004;18(2):132-140
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study evaluated the effects of glucose in human retinal pigment epithelial (RPE) cells to investigate the cause of diabetic retinal complications. Human RPE cells were cultured in media containing 5.5 mM, 11.0 mM, and 16.5 mM D-glucose. The present study performed proliferation and migration assays, and conducted western blotting for the protein expression, as well as RT-PCR for the mRNA expression, of MMP-2 and -9, and TIMP-1 and -2. The results of the western blotting analysis showed that increasing glucose concentration significantly increased the expression of MMP-2 and -9, but significantly decreased the expression of TIMP-1 and -2. Moreover, the RT-PCR results indicated significant increases in the mRNA expression of MMP-2 and -9, as well as of TIMP-1 and -2, by raising glucose concentration. This study provides fundamental data for future research on the mechanism of retinal complication in diabetic patients.
