Identification of Rehmannia glutinosa miR166 Family in Response to Endophytic Fungal Infection and Expression Analysis Under Stresses
10.13422/j.cnki.syfjx.20221618
- VernacularTitle:响应内生真菌侵染的地黄miR166家族鉴定及胁迫下的表达分析
- Author:
Yunhao ZHU
1
;
Jiao XU
1
;
Mengjia ZHANG
1
;
Chengming DONG
1
;
Bo SHI
1
Author Information
1. School of Pharmacy，Respiratory Disease Diagnosis and Treatment and New Drug of Henan Collaborative Innovation Center，Henan University of Chinese Medicine，Zhengzhou 450046，China
- Publication Type:Journal Article
- Keywords:
Rehmannia glutinosa;
miR166;
real-time polymerase chain reaction（Real-time PCR）;
stress;
expression
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(2):133-140
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify the members of the Rehmannia glutinosa miR166 gene family and clarify the response mode under adversity. MethodHigh-throughput sequencing technology was employed to obtain a small RNA database and the miR166 family members of R. glutinosa were screened out. The precursor structures were analyzed by RNAfold. DNAMAN and MEGA were used for conservative and evolutionary analyses， respectively. TargetFinder software was used to predict the target genes of R. glutinosa miR166 family members. The expression of miR166 family members in response to abiotic stress was analyzed by real-time polymerase chain reaction（Real-time PCR）. ResultFive miR166s were identified with precursors possessing complete stem-loop structures. As revealed by sequence alignment results， the precursors and matures were both highly conserved. Forty-eight target genes of miR166s were predicted， which were mainly annotated to the HD-ZIP Ⅲ family transcription factors. The expression characteristics showed that the expression of miR160s was up-regulated after R. glutinosa was infected by endophytic fungi， which was different from the expression of the family members under abiotic stress. The expression level of rgl-miR166b-5p in the drought-flood treatment group and the high-low temperature treatment group was significantly down-regulated compared with that in the control group， and the expression pattern was opposite under the endophytic fungal infection. ConclusionThe results of this study preliminarily clarified the expression patterns of R. glutinosa in response to biotic and abiotic stresses and provided a theoretical basis for future breeding and improvement of R. glutinosa.
