Mechanism of Weiwei Tongtiao Decoction Against Chronic Atrophic Gastritis in Rats： Based on NF-κB Signaling Pathway
10.13422/j.cnki.syfjx.20230499
- VernacularTitle:基于NF-κB信号通路探讨萎胃通调汤对大鼠慢性萎缩性胃炎的作用机制
- Author:
Shaowei YOU
1
;
Xu YI
1
;
Qi ZHAO
1
;
Wensu WANG
1
;
Ling YANG
2
;
Diancheng HE
1
;
Pingzhen TONG
1
;
Xueyong WANG
3
;
Yamei ZHAN
4
Author Information
1. The 2nd Affiliated Hospital of Guizhou University of Traditional Chinese Medicine，Guiyang 550003，China
2. People's Hospital of Changshou Chongqing，Chongqing 401220，China
3. Beijing University of Chinese Medicine，Beijing 102488，China
4. Guizhou Provincial People's Hospital，Guiyang 550002，China
- Publication Type:Journal Article
- Keywords:
chronic atrophic gastritis（CAG）;
Weiwei Tongtiao decoction;
nuclear factor kappa-B（NF-κB） signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(2):88-97
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of Weiwei Tongtiao decoction （WTD） on chronic atrophic gastritis （CAG） rats and the underlying mechanism. MethodA total of 90 SD rats were randomized into normal control group， model group， high-dose， medium-dose， and low-dose WTD groups （18， 9， 4.5 g·kg-1·d-1 WTD， respectively， ig）， and weifuchun control group （0.45 g·kg-1·d-1 weifuchun aqueous solution， ig）， with 15 rats in each group. The N-methyl-N'-nitro-N-nitrosoguanidine （MNNG） was employed to induced CAG in rats. After the modeling （identified by histopathological examination）， the administration began and lasted 12 weeks. Then， gastric mucosa tissues of the rats were collected and stained with hematoxylin and eosin （HE）， and the pathological changes of gastric mucosa were observed under the optical microscope. Real-time PCR， immunohistochemistry （IHC）， and Western blotting were applied to examine the mRNA and protein expression of indexes in nuclear factor kappa-B （NF-κB） signaling pathway in the gastric mucosa of rats. ResultCAG rats showed irregular arrangement and morphology of the inherent glands in gastric mucosa and the glands decreased or disappeared. In addition， inflammatory cell infiltration was observed in the lamina propria of CAG rats， and about 48.4% presented intestinal metaplasia. WTD significantly alleviated the reduction of the glands and intestinal metaplasia in a dose-dependent manner. Compared with the normal control group， the model group demonstrated increase in the mRNA expression of cyclooxygenase-2 （COX-2）， NF-κB p65， interleukin （IL）-1α， IL-1β， IL-10， IL-8α， and IL-8β， and protein expression of COX-2， NF-κB p65， and IL-10 （P<0.01）. WTD and weifuchun lowered the mRNA expression of COX-2， NF-κB p65， IL-1α， IL-1β， IL-10， IL-8α， and IL-8β， and the protein expression of COX-2， NF-κB p65， and IL-10 in gastric mucosa of CAG rats （P<0.01）. The expression of the above indexes after the intervention with high-dose WTD was close to that of the normal control group. ConclusionWTD can improve or even reverse the diseased gastric mucosa of CAG rats， and the mechanism is the likelihood that WTD down-regulates the mRNA and protein expression of the indexes in NF-κB signaling pathway in gastric mucosa of CAG rats.
