Effect of Ermiaosan on Expression of Th17/Treg Cells in DBA/1 Mice with Collagen-induced Arthritis
10.13422/j.cnki.syfjx.20222338
- VernacularTitle:二妙散对DBA/1小鼠胶原诱导型关节炎中Th17/Treg细胞分化的影响
- Author:
Lianhua HE
1
;
Huijie LUAN
1
;
Juan HE
1
;
Xiaocheng WANG
1
;
Yiping HU
1
;
Qingwen WANG
1
Author Information
1. Peking University Shenzhen Hospital, Shenzhen 518036, China
- Publication Type:Journal Article
- Keywords:
Ermiaosan;
collagen-induced arthritis;
T helper 17 （Th17） /regulatory T （Treg） cells;
differentiation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(2):66-72
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of classic prescription Ermiaosan （EMS） on the differentiation of T helper 17 （Th17） /regulatory T （Treg） cells in collagen-induced arthritis （CIA） DBA/1 mice. MethodDBA/1 mice were randomized into normal group， CIA group， EMS （5.4 g·kg-1） group， and methotrexate （MTX，0.5 mg·kg-1） group according to the body weight. DBA/1 mice in CIA group， EMS group， and MTX group were immunized with equal volume of bovine type Ⅱ collagen and complete Freund's adjuvant on the first day and were immunized with equal volume of bovine type Ⅱ collagen and incomplete Freund's adjuvant on the 21st day to induce CIA. On the day of the secondary immunization， intragastric administration started and lasted 28 days （three times/week for MTX group， and once/day for other groups）. The symptoms of CIA mice such as joint redness and swelling were observed from the 22nd day， and the arthritis was scored. After the sampling on the 49th day， synovitis of CIA mice was observed based on hematoxylin-eosin （HE） staining. Double-labeling immunofluorescence （IF） method was used to detect the expression of Th17 cell marker IL-17 and Treg cell marker forkhead transcription factor P3 （FoxP3） in CD4+T cells in CIA mouse joints. The proportion of Th17 and Treg cells in the spleen and lymph nodes of mice was detected by flow cytometry. ResultCompared with the normal group， CIA group had obvious synovitis， disordered joint structure， severely damaged articular cartilage and bone， serious bone erosion （P<0.01）， high Th17/Treg value in joint tissue （P<0.01）， high proportion of Th17 cells in spleen and lymph nodes （P<0.01）， and low proportion of Treg cells （P<0.01）. Compared with CIA group， EMS group and MTX group had normal joint structure， mild bone erosion and bone destruction， complete and smooth joint surface， low Th17/Treg value （P<0.01）， low proportion of Th17 cells in spleen and lymph nodes （P<0.01）， and high proportion of Treg cells in spleen and lymph nodes （P<0.01）. ConclusionEMS regulates the balance of Th17/Treg， inhibits the expression of Th17 cells， and promotes the expression of Treg cells in CIA mice， thereby exerting therapeutic effect on RA.
