Effect of Guizhi Shaoyao Zhimutang on Cartilage Destruction and JAK2/STAT3 Signaling Pathway in Mice with Collagen-induced Arthritis
10.13422/j.cnki.syfjx.20221403
- VernacularTitle:桂枝芍药知母汤对胶原诱导性关节炎小鼠软骨破坏及JAK2/STAT3信号通路的影响
- Author:
Nan LI
1
;
Haixin YANG
1
;
Shan ZENG
2
;
Qingyu MA
1
;
Jiaxu CHEN
1
Author Information
1. School of Traditional Chinese Medicine，Jinan University，Guangzhou 510632，China
2. The First Affiliated Hospital of Jinan University，Guangzhou 510632，China
- Publication Type:Journal Article
- Keywords:
Guizhi Shaoyao Zhimutang;
rheumatoid arthritis;
cartilage destruction;
collagen-induced arthritis （CIA）;
Janus kinase 2/signal transducer and activator of transcription 3 （JAK2/STAT3） signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(2):52-58
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effect of Guizhi Shaoyao Zhimutang （GSZMD） on cartilage destruction in mice with collagen-induced arthritis （CIA） and its mechanism. MethodThirty-six DBA/1 mice in SPF grades were randomly divided into 6 groups， namely， the normal group， the model group， the methotrexate （MTX） group， the low-dose GSZMD group， the medium-dose GSZMD group， and the high-dose GSZMD group. Except the normal group， mice in the other 5 groups were used to establish the model of CIA by secondary immunization. The mice were given normal saline， MTX （1.5 mg·kg-1， 2 times a week）， and low， medium， and high-doses GSZMD （6.3， 12.6， 25.2 g·kg-1·d-1） by intragastric administration on the day of the onset of hind limb swelling for 4 weeks. The changes in the degree of foot swelling of mice in each group were observed and recorded. The content of matrix metalloproteinase （MMP）-1， MMP-3， MMP-9， and MMP-13 in serum was determined by enzyme-linked immunosorbent assay （ELISA）. The pathological changes in the ankle joint were observed by hematoxylin-eosin （HE） staining， and the cartilage destruction was observed by red fast green staining. The protein expression of the Janus kinase 2/signal transducer and activator of transcription 3 （JAK2/STAT3） signaling pathway in ankle joints were detected by Western blot. ResultAs compared with the normal group， the degree of foot swelling， the content of MMP-1， MMP-3， MMP-9， and MMP-13 in serum and the expression levels of phosphorylation （p）-JAK2/JAK2 and p-STAT3/STAT3 in ankle joints of the model group were increased （P<0.01）， and the joint damage was aggravated. As compared with the model group， the degrees of foot swelling of the mice in the MTX group and the low， medium， and high-dose GSZMD group were reduced （P<0.05， P<0.01）， and the content of MMP-1， MMP-3， MMP-9， and MMP-13 in serum was decreased （P<0.05， P<0.01）. The pathological joint damage was alleviated， and the expression levels of p-JAK2/JAK2 and p-STAT3/STAT3 in ankle joints were decreased in the MTX group and GSZMD groups （P<0.05， P<0.01）. ConclusionGSZMD can reduce the degree of joint swelling in mice with CIA， inhibit the expressions of MMP-1， MMP-3， MMP-9， and MMP-13， and alleviate the destruction of articular cartilage. Its mechanism is related to the JAK2/STAT3 signaling pathway.
