Bone Marrow Mesenchymal Stem Cells' Transformation into Nerve Cells Induced by Basic Fibroblast Growth Factor-Chitosan Carrier

VernacularTitle:碱性成纤维细胞生长因子-壳聚糖载体诱导成年大鼠骨髓间充质干细胞向神经细胞的转化
Author: Xiaomeng TU ; Hongmei DUAN ; Jiasheng RAO ; Zhaoyang YANG ; Xiaoguang LI
Publication Type:Journal Article
Keywords: bone marrow mesenchymal stem cell, basic fibroblast growth factor, chitosan, nerve transformation, neural stem cells, neurons, astrocytes, rats
From: Chinese Journal of Rehabilitation Theory and Practice 2013;19(10):916-921
CountryChina
Language:Chinese
Abstract: Objective To explore the induction of basic fibroblast growth factor (bFGF)-chitosan carrier transforming the adult rat bone marrow mesenchymal stem cells (rMSCs) into nerve cells. Methods rMSCs were detected qualitatively and counted quantitatively by immunohistochemistry after they were induced into nerve cells, such as neural stem cells neurons and astrocytes. The methyl thiazolyl tetrazolium (MTT) chromometry assay was carried out to determine the cell viability. Results rMSCs induced by bFGF-chitosan carrier expressed neural stem cell marker nestin, neuron marker β-tubulin Ⅲ and astrocytes marker glial fibrillary acidic protein (GFAP). Nestin expressed more in the bFGF-chitosan group, and reached its maximum (49.40%) at the 9th day. Conclusion bFGF-chitosan carrier can induce the adult rMSCs differentiate into neural stem cells in a high proportion.