Effect of combined sodium hyaluronate and carboxymethyl cellulose on ocular surface in rat dry eye model.
10.14405/kjvr.2016.56.3.155
- Author:
Jong Gab MOON
1
;
Sae Kwang KU
;
Young Sam KWON
Author Information
1. Department of Veterinary surgery, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea. kwon@knu.ac.kr
- Publication Type:Original Article
- Keywords:
carboxymethyl cellulose;
dry eye;
rat;
sodium hyaluronate
- MeSH:
Animals;
Carboxymethylcellulose Sodium*;
Conjunctiva;
Cornea;
Corneal Stroma;
Epithelium;
Epithelium, Corneal;
Goblet Cells;
Hyaluronic Acid*;
Occupations;
Rats*;
Sodium*
- From:Korean Journal of Veterinary Research
2016;56(3):155-160
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was conducted to evaluate three different mixed formulations of sodium hyaluronate (SH) and carboxymethyl cellulose (CMC) using a low-humidity air flow-induced rat dry eye model and determine the most suitable mixture. The total thickness of the cornea, corneal epithelial thickness, corneal stroma thickness, damaged corneal epithelium percentage region, thickness of the bulbar conjunctiva epithelium, number of goblet cells, goblet cell occupation percentage region, and damaged bulbar conjunctiva epithelium percentage region were measured by histomorphological evaluation. After 5 h exposure to drying airflow, the thickness of the cornea and conjunctiva was decreased with desquamation of the corneal and conjunctiva epithelium. However, these dry eye symptoms were markedly inhibited by treatment with the reference and test formulations. More favorable effects on decreased thickness were detected in response to the CMC than the SH. However, SH had a greater protective effect against corneal and conjunctiva epithelial damage. The application of a mixture of 0.1% SH and 0.2% CMC showed more favorable effects on the corneal and conjunctival damage and the stabilization of the ocular surface than SH or CMC alone.
