Rapid determination of vitamin D3 in poultry eggs and their products byon-line column switching liquid chromatography
10.19485/j.cnki.issn2096-5087.2022.12.019
- Author:
Yin WANG
;
Baifen HUANG
;
Xiaodong PAN
- Publication Type:Journal Article
- Keywords:
on-line column switching;
liquid chromatography;
vitamin D3;
poultry egg
- From:
Journal of Preventive Medicine
2022;34(12):1289-1294
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an on-line column switching liquid chromatography for quantitative determination of vitamin D3 in poultry eggs and their products.
Methods:Antioxidant-protected samples were saponified, extracted with a mixture of ethyl acetate-n-hexane mixture (6︰4, v/v) and subjected for determination using two-dimensional liquid chromatography. One-dimensional chromatography was performed with the UniChiral® OD-5H column (4.6 mm × 100 mm, 5 μm), acetonitrile: methanol (75︰25, v/v) and water as a mobile phase for gradient elution at a flow rate of 1.00 mL/min to complete vitamin D purification, and two-dimensional chromatography was performed with the Agilent Eclipse PAH column (2.1 mm × 100 mm, 3.5 μm, column temperature of 35 ℃), detection wavelength of 264 nm for separation and determination of vitamin D3 and internal standard vitamin D2 with the internal standard method.
Results:Vitamin D3 had a good linear relationship within the range of 2.5 to 100 ng/mL, with r2 of >0.999. The mean spiked recovery rate of vitamin D3 was 101.23% to 102.08% in egg yolk powder, with relative standard deviation (RSD) of 3.91% to 5.85%, detection limit of 1.5 μg/100 g, and quantitative limit of 4.9 μg/100 g, and the limits of detection and quantitation of vitamin D3 were 0.15 μg/100 g and 0.49 μg/100 g in whole egg liquids.
Conclusions:The method is simple, rapid, highly accurate, sensitive and reproducible, which is suitable for rapid and quantitative determination of vitamin D3 in poultry eggs and their products.
- Full text:在线柱切换-液相色谱法快速测定禽蛋及其制品中的维生素D3.pdf
