Hens immunized with live attenuated Salmonella strains expressing virulence-associated genes in avian pathogenic Escherichia coli passively transfer maternal antibodies to chicks.
10.14405/kjvr.2016.56.3.167
- Author:
Gayeon WON
1
;
John Hwa LEE
Author Information
1. College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea. johnhlee@jbnu.ac.kr
- Publication Type:Original Article
- Keywords:
avian pathogenic Escherichia coli;
immunity;
passive antibody transfer;
protection;
vaccine
- MeSH:
Antibodies*;
Egg Yolk;
Eggs;
Escherichia coli*;
Escherichia*;
Immunization;
Immunization, Passive;
Immunoglobulin A;
Immunoglobulin A, Secretory;
Immunoglobulin G;
Mortality;
Ovum;
Plasma;
Plasmids;
Salmonella typhimurium;
Salmonella*
- From:Korean Journal of Veterinary Research
2016;56(3):167-176
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated whether maternal antibodies (mAbs) elicited by dams immunized with recombinant vaccine candidates against avian pathogenic Escherichia coli (APEC) can passively confer protective immunity to chicks. In the present study, pBP244 plasmids carrying selected antigens of APEC were transformed into Salmonella Typhimurium JOL912, which was used as a vaccine candidate against APEC. The hens were immunized with the vaccine candidates using prime or booster doses. The levels of IgG and sIgA specific to the selected antigens increased significantly following prime immunization. To evaluate the persistence of passively transferred mAbs, the levels of IgY and IgA were determined in egg yolks and whites, respectively. The eggs from the immunized group showed consistently increased levels of IgY and IgA until week 16 post-laying (PL) and week 8 PL, respectively, relative to the control group. The presence of mAbs was observed in chicks that hatched from the hens, and titers of plasma IgY were consistently raised in those from the immunized hens by day 14 post-hatching. Further, chicks from the immunized hens were protected from challenge with a virulent APEC strain, whereas those from non-immunized hens showed acute mortality.
