Differential Analysis of Flavonoid-regulated Genes in Two Cultivars of Angelica sinensis Based on Hybrid Sequencing Strategy
10.13422/j.cnki.syfjx.20221514
- VernacularTitle:基于混合测序策略的两品种当归中类黄酮调控基因差异分析
- Author:
Li XU
1
;
Tiantian ZHU
1
;
Ling JIN
1
;
Shuqi KANG
1
;
Minghui ZHANG
1
;
Yuanyuan WANG
1
Author Information
1. Gansu University of Chinese Medicine， Lanzhou 730000， China
- Publication Type:Journal Article
- Keywords:
Angelica sinensis;
full-length transcriptome;
RNA sequencing technology;
flavonoid biosynthesis;
differentially expressed genes
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(1):141-147
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo compare the transcriptional levels of two cultivars （Mingui 1 and Mingui 2） with different stem and leaf colors. MethodThe fresh leaves with petioles and the upper stems from Angelica sinensis with two colors were selected as the material， and the hybrid sequencing strategy was used. The technology at the transcriptome was used to build the non-reference full-length transcript library of A. sinensis， and the RNA-seq technology was used to analyze differentially expressed genes of the two cultivars， reusing a public database for biology function annotation and fine classification of differentially expressed genes. The main candidate genes regulating color differences between stems and leaves of A. sinensis were screened out. ResultThe sequencing results of A. sinensis transcripts were good and the quality of the sequencing data was high. The 34 528 full-length transcripts were annotated into 33 947， 33 241， 29 150， and 22 601 in the Non-redundant Protein Sequence Database （NR）， Kyoto Encyclopedia of Genes and Genomes （KEGG）， SwissProt， and Clusters of Orthologous Groups for Eukaryotic Complete Genomes （KOG）， respectively. The 705 differentially expressed genes of the two cultivars with biological and molecular functions were divided into 11 categories， which were mainly enriched in the primary metabolism （17.87%）， stress response （14.47%）， and secondary metabolism （11.49%）. The differentially expressed genes related to colors were mainly concentrated in the flavonoid biosynthesis pathway. ConclusionThe main reason for the color differences of the stems and leaves in two cultivars of A. sinensis may be related to the expression differences of genes regulating flavonoid biosynthesis， which lays a foundation for subsequent functional verification and further clarification of the relationship with the main pharmacodynamic components of A. sinensis.
