Analysis of Prototype Components and Metabolites from Bergeniae Scopulosae Rhizoma Absorbed into Blood by UHPLC-Q Exactive Focus MS/MS
10.13422/j.cnki.syfjx.20220554
- VernacularTitle:秦岭岩白菜入血成分及其代谢产物的UHPLC-Q Exactive Focus MS/MS分析
- Author:
Hui REN
1
;
Wenjing LU
1
;
Xiaomin CUI
1
;
Jing HU
1
;
Ning LI
1
;
Tong QU
1
;
Zhiyong CHEN
1
Author Information
1. Shaanxi Academy of Traditional Chinese Medicine，Xi'an 710003，China
- Publication Type:Journal Article
- Keywords:
Bergeniae Scopulosae Rhizoma;
Bergenia scopulosa;
ultra-high performance liquid chromatography-quadrupole/electrostatic field orbital trap high resolution mass spectrometry （UHPLC-Q Exactive Focus MS/MS）;
prototype components;
metabolites;
phenolic acids;
C-glycoside
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(1):113-122
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the blood-transition constituents of Bergeniae Scopulosae Rhizoma extract based on the serum pharmacochemical method. MethodUltra-high performance liquid chromatography-quadrupole/electrostatic field orbital trap high resolution mass spectrometry （UHPLC-Q Exactive Focus MS/MS） technique was used to compare the chromatogram differences of Bergeniae Scopulosae Rhizoma extract， blank serum and drug-containing serum， according to the retention time， relative molecular weight and the ratio with primary and secondary ion fragments provided by MS， the prototype components and metabolites of Bergeniae Scopulosae Rhizoma extract were analyzed in serum of rats after oral administration. The detection conditions were as follows：the mobile phase of methanol （A）-0.1% formic acid solution （B） for elution gradient （0-40 min， 5%-95%A； 40-45 min， 95%A）， the flow rate of 0.3 mL·min-1， the injection volume of 1 µL， heated electrospray ionization， detection range of m/z 80-1 200， positive and negative ion scanning modes. ResultAfter oral administration， a total of 35 components were detected in the serum， of which 6 were prototype components and 29 were metabolites of prototype components. The 6 prototype components were identified as arbutin， mallonanoside A， bengenin， ardimerin， salicylic acid and ellagic acid. The 29 metabolites were mainly metabolites from compound containing structural units such as gallic acid， mallonanoside A， bengenin， catechin， and ellagic acid， and their metabolic pathways were mainly glucuronidation， sulfation， methylation and others， which by phase Ⅱ metabolism. ConclusionThe components absorbed into the blood and their metabolites may be the pharmacodynamic components of Bergeniae Scopulosae Rhizoma. Among them， mallonanoside A， bengenin and ellagic acid can not only enter the blood as prototype components， but also exert their efficacy through phase Ⅱ metabolism. Most of the other chemical components in Bergeniae Scopulosae Rhizoma may be transformed into simple structures such as gallic acid， bengenin and catechin by hydrolysis， and then further metabolized by phase Ⅱ.
