Regulation of Danshen Zexie Decoction on AMPK/Nrf2 Signaling Pathway in Rats with Metabolic-associated Fatty Liver Disease
10.13422/j.cnki.syfjx.20222244
- VernacularTitle:丹参泽泻汤对代谢相关脂肪性肝病大鼠AMPK/Nrf2信号通路的调节作用
- Author:
Yaning BIAO
1
;
Xinqiao CHU
2
;
Muqing ZHANG
3
;
Chenxu LIU
1
;
Cheng SHI
1
;
Sitong LI
1
;
Yixin ZHANG
1
Author Information
1. Application Technology Research and Development Center of Traditional Chinese Medicine （TCM） in Hebei Universities， International Joint Research Center on Resource Utilization and Quality Evaluation of TCM of Hebei Province， School of Pharmacy， Hebei University of Chinese Medicine， Shijiazhuang 050200， China
2. Guang'anmen Hospital， China Academy of Chinese Medical Sciences， Beijing 100053， China
3. Hebei Provincial Hospital of TCM， Shijiazhuang 050011， China
- Publication Type:Journal Article
- Keywords:
metabolic-associated fatty liver disease;
Danshen Zexie decoction;
oxidative stress;
adenosine monophosphate-activated protein kinase （AMPK）， nuclear factor E2-related factor 2 （Nrf2）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(1):67-74
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the efficacy and mechanism of Danshen Zexie decoction in treating the rats with metabolic-associated fatty liver disease （MAFLD）. MethodSixty male SD rats were randomized into control group， model group， essentiale （polyene phosphatidylcholine capsules， 0.144 g·kg-1） group， and low-， medium-， and high-dose Danshen Zexie decoction （1.16， 2.32， and 4.64 g·kg-1， respectively） groups. The rat model of MAFLD was established with high fat diet， and 8-week therapy started at the induction of MAFLD. The serum levels of total cholesterol （TC）， triglyceride （TG）， alanine aminotransferase （ALT）， and aspartate aminotransferase （AST）， as well as the liver levels of TC， TG， free fatty acid （FFA）， superoxide dismutase （SOD）， glutathione （GSH）， and malondialdehyde （MDA）， were measured. The pathological changes of liver were observed by hematoxylin-eosin （HE） staining and oil red O staining， and the steatosis score and oil red O staining area were calculated. The level of reactive oxygen species （ROS） in the liver was detected by immunofluorescence. The protein levels of adenosine monophosphate-activated protein kinase （AMPK）， p-AMPK， nuclear factor E2-related factor 2 （Nrf2）， glutamate-cysteine ligase catalytic subunit （GCLC）， glutamate-cysteine ligase modifier subunit （GCLM）， and nicotinamide adenine dinucleotide phosphate： quinine oxidoreductase （NQO1） in the liver were determined by Western blot. ResultCompared with control group， the modeling of MAFLD elevated the levels of TC， TG， ALT， and AST in the serum and TC， TG， FFA， MDA， and ROS in the liver （P<0.01）， lowered the levels of SOD and GSH and down-regulated the protein levels of phosphorylation（p）-AMPK， Nrf2， GCLC， GCLM， and NQO1 in the liver （P<0.05， P<0.01）. Further， a large number of lipid droplet vacuoles and balloon-like lesions appeared in the hepatocytes， and the steatosis score and oil red O staining area increased in the model group （P<0.01）. Compared with model group， Danshen Zexie decoction lowered the levels of TC， TG， ALT， and AST in the serum and TC， TG， FFA， MDA， and ROS in the liver （P<0.01）， increased the levels of SOD and GSH and up-regulated the protein levels of p-AMPK， Nrf2， GCLC， GCLM， and NQO1 in the liver （P<0.05， P<0.01）. Moreover， the decoction alleviated the degree of liver steatosis （P<0.05， P<0.01）. ConclusionDanshen Zexie decoction protects against MAFLD by activating AMPK/Nrf2 signaling pathway and suppressing oxidative stress.
