Mechanism of Toxicity Reduction of Psoralidin Combined with Echinatin
10.13422/j.cnki.syfjx.20221502
- VernacularTitle:补骨脂定-刺甘草查尔酮配伍减毒机制
- Author:
Tingting LIU
1
;
Longxin LIANG
2
;
Guang XU
2
;
Xiaohe XIAO
2
;
Yanling ZHAO
2
;
Zhaofang BAI
2
Author Information
1. School of Pharmacy，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China
2. The Fifth Medical Center of Chinese People's Liberation Army General Hospital，Beijing 100039，China
- Publication Type:Journal Article
- Keywords:
psoralidin;
echinatin;
toxicity reduction by combination;
immunomodulation;
inflammasome
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(1):45-51
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a model of inflammasome activation induced by psoralidin based on bone marrow-derived macrophages （BMDM） in mice， and to explore the immunomodulatory effects of psoralidin combined with echinatin. MethodLipopolysaccharide （LPS） and psoralidin were used to activate inflammasomes， and after 4 h LPS stimulation， echinatin （40 μmol·L-1） was administered for pre-protection for 1 h， followed by stimulation with psoralidin （10， 20， 40 μmol·L-1） for 4 h. The protein expression of Caspase-1 p20 in cell supernatant and precursor （pro）-Caspase-1 and pro-interleukin（IL）-1β in cell lysate were simultaneously detected by Western blot. Enzyme-linked immunosorbent assay （ELISA） was adopted to determine the content of IL-β and TNF-α in the supernatant of BMDM. ResultWestern blot revealed that compared with the conditions in the control group， the maturation of psoralidin-induced pro-Caspase-1 and the secretion of IL-1β was inhibited by echinatin （P<0.05，P<0.01）. ELISA showed that the production of IL-1β and TNF-α was enhanced by psoralidin of different concentrations （P<0.05，P<0.01） compared with the condition in the control group. In addition， compared with the psoralidin group， the psoralidin combined with echinatin group reduced the secretion of IL-1β and TNF-α （P<0.01）. ConclusionEchinatin could significantly inhibited the excessive immune-inflammatory response mediated by psoralidin， and thus achieve the effect of toxicity reduction. The present study explored the toxicity-reducing effect of psoralidin combined with echiantin， providing a basis for safe clinical application.
