Comparison of parentage testing results from several institutes in Korea.
- Author:
Jin Young SON
1
;
Soong Deok LEE
;
Gil Ro HAN
;
Myun Soo HAN
;
Seung Bum HONG
;
Kwang Man WOO
;
Seung Hwan LEE
;
Kyoung Jin SHIN
;
Yun Seok YANG
;
Jong Tae PARK
;
Eun Young KIM
;
Yeon Bo CHUNG
Author Information
1. Department of Forensic Medicine, Seoul National University, Korea. sdlee@snu.ac.kr
- Publication Type:Original Article
- Keywords:
collaborative work;
parentage testing;
Korea;
Standardization;
Profiling
- MeSH:
Academies and Institutes*;
Child;
DNA Fingerprinting;
Humans;
Korea*;
Minisatellite Repeats
- From:Korean Journal of Legal Medicine
2002;26(1):68-74
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Collaborative work using same samples for the parentage testing, which was intended to see the status and the quality of several DNA typing laboratories in Korea, was described. Samples were consisted of two sets, one was a trio case and the other was a deficient case with two children. Samples were sent to six laboratories, among which five submitted the result. Each laboratory had used different number and set of STR loci using 14 - 23 loci, and total 33 different loci were used. Only one VNTR locus, D1S80 was included and all the remaining were STR loci. The loci included in the commercial kits were used more frequently. One laboratory had used Korean-made commercial kits. All the laboratories gave the same results about the parentage, although results for one locus were not the same through different laboratories. There existed minor difference in the PI calculation, especially in the statistical parameters such as allelic frequences, which might gave confusion to users of the results who were not familiar with the test. Necessity about the standardization and profiling data were discussed.
