Rapid identification of chemical constituents in Abrus cantoniensis Hance by UPLC-Q-TOF/MS combined with UNIFI platform
- VernacularTitle:UPLC-Q-TOF/MS技术结合UNIFI平台快速鉴定鸡骨草化学成分
- Author:
Zhonghui GUO
1
;
Xueying ZHENG
1
;
Jie LIANG
1
;
Yong TAN
1
;
Chunping QIN
1
;
Jiacheng GUO
1
;
Yaohua LI
2
;
Zhiying WEI
2
;
Kuikui CHEN
1
Author Information
1. School of Pharmacy，Guangxi University of Chinese Medicine，Nanning 530200，China
2. Teaching Experiment Training Center，Guangxi University of Chinese Medicine，Nanning 530200，China
- Publication Type:Journal Article
- Keywords:
Abrus cantoniensis Hance;
chemical constituents;
identification;
UPLC-Q-TOF/MS;
UNIFI platform
- From:
China Pharmacy
2022;33(23):2852-2857
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish an analytical method based on ultra-performance liquid chromatography-quadrupole-time- of-flight mass spectrometry （UPLC-Q-TOF/MS） combined with UNIFI platform for rapid identification of the chemical constituents in Abrus cantoniensis Hance. METHODS The chromatographic separation was performed on ACQUITY PRM HSS T3 FIT column for gradient elution with the mobile phase consisted of 0.1% formic acid aqueous solution-acetonitrile. The flow rate was 0.3 mL/min， and injection volume was 2 μL. Electrospray ionization was used to collect the mass spectrometry data of the chemical constituents of A. cantoniensis Hance with full information tandem mass spectrometry technology in positive and negative ion modes. The chemical constituent database of A. cantoniensis Hance was established. Targeted and non-targeted analyses were conducted based on UNIFI platform， and chemical constituents were further identified in combination with accurate molecular mass， secondary fragment ion information and equivalence with reference substances and literature data， etc. RESULTS Totally 46 compounds of A. cantoniensis Hance were successfully identified， including 19 flavonoids， 8 triterpenoids， 3 alkaloids， 5 organic acids and 11 other components. Among them， 11 compounds were firstly found in A. cantoniensis Hance， and 9 compounds were confirmed by reference substance. CONCLUSIONS The analytical method based on UPLC-Q-TOF/MS combined with UNIFI platform can quickly identify the chemical constituents of A. cantoniensis Hance. Flavonoids and triterpenes are the main components in A. cantoniensis Hance.
