Study on pharmacological components and mechanism of Qingre huoxue decoction in improving myocardial ischemia-reperfusion injury
- VernacularTitle:清热活血汤改善大鼠心肌缺血再灌注损伤的药效成分及作用机制研究
- Author:
Rui LI
1
;
Shuliang JI
2
;
Jiechun ZHANG
3
;
Zhizhong SUN
2
;
Xiaodan YANG
2
;
Yude LIU
1
;
Zaoyuan KUANG
4
;
Lijin QING
1
;
Wei WU
1
Author Information
1. Dept. of Cardiovascular Disease，the First Affiliated Hospital of Guangzhou University of Chinese Medicine，Guangzhou 440100，China
2. The First Clinical School of Medicine，Guangzhou University of Chinese Medicine，Guangzhou 440100，China
3. Dept. of Respiration，the Affiliated TCM Hospital of Guangzhou Medical University，Guangzhou 440100，China
4. College of Basic Medicine，Guangzhou University of Chinese Medicine，Guangzhou 440100，China
- Publication Type:Journal Article
- Keywords:
Qingre huoxue decoction;
myocardial ischemia-reperfusion injury;
pharmacological components;
mechanism
- From:
China Pharmacy
2022;33(23):2845-2851
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the pharmacological components and mechanism of Qingre huoxue decoction in improving myocardial ischemia-reperfusion injury（MIRI）. METHODS Forty-two rats were randomly divided into sham operation group （normal saline）， model group （normal saline）， Qingre huoxue decoction low-dose， medium-dose and high-dose groups （4.94， 9.88， 19.79 g/kg），Qingre huoxue decoction drug-containing serum group （19.79 g/kg） and blank serum group （normal saline）， with 6 rats in each group. Each group was given corresponding drug/normal saline intragastrically， once a day， for consecutive 2 weeks. Twelve hours after last administration， except for serum groups， MIRI model was induced in other groups （only threading without ligation in sham operation group）. After modeling， cardiac histopathology was observed and apoptosis level was detected. UPLC-MS was used to analyze the samples in Qingre huoxue decoction drug-containing serum group and blank serum group. The main pharmacological components were screened with the help of relevant databases. Multivariate statistical methods were used to analyze the differential metabolites and related metabolic pathways. Validation test was performed based on oxidative stress indicators. RESULTS Qingre huoxue decoction could improve the pathological injury of cardiac tissue and decrease apoptosis rate of cardiac cells in MIRI model rats （P＜0.05）. Qingre huoxue decoction drug-containing serum contained 20 main pharmacological components such as baicalin， succinic acid， baicalein， cryptotanshinone， isoferulic acid， protocatechuic aldehyde. Qingre huoxue decoction could significantly up-regulate the levels of 15 metabolites including L-arginine， L-arginine， citric acid， glutathione， β-D- glucose and L-carnitine， and down-regulated the levels of 14 metabolites including arachidonic acid， 3-phosphate-d-glycerol phosphate， linoleic acid， docosahexaenoic acid， phosphatidylcholine and lysophosphatidylcholine （P＜0.05）. These metabolites were mainly involved in energy metabolism， inflammatory injury， oxidative stress and autophagy. Results of validation tests showed that Qingre huoxue decoction could significantly reduce the levels of malondialdehyde， and increased the levels of superoxide dismutase （except for low-dose group） and glutathione peroxidase significantly （P＜0.05）. CONCLUSIONS Qingre huoxue decoction can improve the injury of cardiac tissue in MIRI model rats. Its pharmacological components include baicalin， cryptotanshinone， isoferulic acid， protocatechualdehyde， etc. Furthermore， it may play a protective role in MIRI by improving myocardial energy metabolism， down-regulating oxidative stress， inhibiting inflammation infiltration.
