Study on mechanism of action of Fuke duanhongyin capsule on the improvement of dysfunctional uterine bleeding in rats
- VernacularTitle:妇科断红饮胶囊改善大鼠功能失调性子宫出血的作用机制研究
- Author:
Ying HE
1
;
Yonggen LING
2
;
Hongqing ZHAO
1
;
Mengyao WU
2
;
Lu BAI
2
;
Qian CHEN
1
;
Haohan ZHOU
1
;
Yuhong WANG
1
Author Information
1. Science and Technology Innovation Center，Hunan University of Chinese Medicine，Changsha 410208，China
2. Zhuzhou Qianjin Pharmaceutical Co.，Ltd.，Hunan Zhuzhou 412000，China
- Publication Type:Journal Article
- Keywords:
Fuke duanghongyin capsule;
dysfunctional uterine bleeding;
epidermal growth factor;
epidermal growth factor
- From:
China Pharmacy
2022;33(23):2840-2844
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the mechanism of Fuke duanhongyin capsule in improving dysfunctional uterine bleeding （DUB） in rats. METHODS Pregnant SD rats were randomly divided into blank control group （distilled water）， model control group （distilled water） and Fuke duanghongyin capsule group （1.296 g/kg）， with 6 rats in each group. Except for blank control group， DUB model was established in other groups. After modeling， they were given relevant medicine/distilled water 10 mL/kg intragastrically， once a day， for consecutive 14 d. After medication， uterine index， ovary index and hemorheology indexes （whole blood high shear relative index， whole blood low shear relative index， erythrocyte aggregation index， Carson viscosity） of rats were all determined. The pathological changes of uterus and ovary tissues were observed； mRNA relative expression levels of epidermal growth factor （EGF）， hypoxia-inducible factor 1α （HIF-1α）， epidermal growth factor receptor （EGFR） and serine/ threonine-protein kinase 1（AKT1）， and protein relative expression levels of EGF and EGFR in uterine tissue were all measured. RESULTS Compared with model control group， the uterine index， ovary index， erythrocyte aggregation index were all decreased significantly in Fuke duanghongyin capsule group （P＜0.05 or P＜0.01）； whole blood high shear relative indexes， mRNA relative expression levels of EGF， HIF-1α and EGFR and protein relative expression levels of EGF and EGFR in uterine tissue were all increased significantly （P＜0.05 or P＜0.01）. The pathological changes of ovary and uterus were improved. CONCLUSIONS Fuke duanhongyin capsule can improve DUB in rat， the mechanism of which may be related to the activation of EGF-EGFR signaling pathway.
