Study on intervention effect of Bushen qiangshen tablet on polycystic ovary syndrome model rats and its mechanism
- VernacularTitle:补肾强身片对多囊卵巢综合征模型大鼠的干预作用及机制研究
- Author:
Minghao ZHANG
1
;
Yiying GAO
1
;
Wenxia DONG
1
;
Pengkun XUE
1
;
Weiyang MA
1
;
Dawei ZHANG
2
;
Liya MA
3
Author Information
1. Medical College，Henan University of Traditional Chinese Medicine，Zhengzhou 450046，China
2. Dept. of Obstetrics and Gynecology，the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine，Zhengzhou 450008，China
3. Clinical Skills Training Center，Henan University of Traditional Chinese Medicine，Zhengzhou 450046，China
- Publication Type:Journal Article
- Keywords:
Bushen qiangshen tablet;
polycystic ovary syndrome;
PI3K/Akt/mTOR signaling pathway;
PI3K/Akt/GLUT4
- From:
China Pharmacy
2022;33(21):2632-2637
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the intervention effect of Bushen qiangshen tablet on polycystic ovary syndrome （PCOS） model rats and its mechanism . METHODS Totally 50 rats were given letrozole suspension （1 mg/kg，once a day ，for consecutive 21 d） instragastrically to induce PCOS model . Model rars were randomly divided into model group ，positive control group （Ethinylestradiol and cyproterone acetate tables 0.2 mg/kg+Metformin hydrochloride tables 230 mg/kg），Bushen qiangshen tablet low-dose，medium-dose and high -dose groups （189，378，756 mg/kg），with 10 rats in each group . Another 10 healthy rats were included in normal group . Each group was given the corresponding drugs ，once a day ，for consecutive 30 d. Twenty-four hours after the last administration ，serum levels of estrogen （E2），testosterone（T），gonadotropin-releasing hormone （GnRH），follicle- stimulating hormone （FSH）and luteinizing hormone （LH）were measured . The ovary index was calculated ，and pathological changes of ovary were observed . The protein expressions of phosphoinositide 3-kinase（PI3K），phosphorylated protein kinase B （p- Akt），phosphorylated mammalian target of rapamycin （p-mTOR）and glucose transporter 4（GLUT4）in ovary were detected ，and mRNA expressions of PI 3K，Akt，mTOR and GLUT 4 in ovary were detected . RESULTS Compared with normal group ，ovarian index and ovarian cystic disease score were significantly increased in model group （P＜0.05），and serum levels of T ，GnRH and LH were significantly increased （P＜0.05）；serum levels of FSH and E 2，protein expressions of PI 3K，p-Akt，p-mTOR and GLUT4，and mRNA expression of PI 3K，AKT，mTOR and GLUT 4 in ovarian tissue were all significantly decreased in the model group （P＜0.05）；in ovarian tissue ，the number of atresia follicles and non -cumulus cystic follicles increased ，and the number of granulosa cell layers decreased . Compared with model group ， above indexes of Bushen qiangshen tablet medium-dose and high -dose g roups were reversed significantly 86550201。E-mail：mary868@163.com （P＜0.05），and most above indexes of low -dose group were reversed significantly （P＜0.05），the pathological changes of ovarian tissue were improved to varying degrees . CONCLUSIONS Bushen qiangshen tablet can regulate the secretion of sex hormones in PCOS model rats and improve ovarian cystic lesions . Its mechanism may be related to the upregulation of PI 3K/Akt/mTOR and PI 3K/Akt/GLUT4 signaling pathways .
