Establishment of fingerprint，chemical pattern recognition and multi-component content determination of Compound huiqin granules
- VernacularTitle:复方茴芹颗粒的指纹图谱建立、化学模式识别及多成分含量测定
- Author:
Huifeng LI
1
;
Xiangpeng KONG
1
;
Shuang MENG
2
;
Hui LI
1
;
Miaorong PEI
1
Author Information
1. College of Chinese Medicine and Food Engineering，Shanxi University of Traditional Chinese Medicine，Shanxi Jinzhong 030619，China
2. Central Laboratory，Shanxi Hospital of Integrated Traditional and Western Medicine，Taiyuan 030013，China
- Publication Type:Journal Article
- Keywords:
compound huiqin granules;
high-performance liquid chromatography method;
fingerprint;
content determination
- From:
China Pharmacy
2022;33(21):2627-2631
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the high -performance liquid chromatography （HPLC）fingerprint of Compound huiqin granules and analyze it with chemical pattern recognition ，and determine the contents of 8 active components in the granules . METHODS Using puerarin as reference peak ，the fingerprints of 10 batches of Compound huiqin granules were drawn by HPLC method. Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine （2012 edition）was used to evaluate the similarity and identify common peaks ；SPSS 22.0 and SIMCA 14.0 software were used for cluster analysis and principal component analysis . HPLC method was used to determine the contents of 8 active components ，such as chlorogenic acid ， puerarin，3′-methoxypuerarin，daidzin，luteolin-7-O-β-D-glucuronide，isochlorogenic acid A ，and apigenin -7-O-β-D-glucuronide and isochlorogenic acid C . RESULTS There were 26 common peaks in the fingerprints of 10 batches of Compound huiqin granules，the similarity was greater than 0.900，and 11 peaks were identified ，which were gallic acid ，neochlorogenic acid ， chlorogenic acid ，puerarin，3′-methoxypuerarin，daidzin，luteolin-7-O-β-D glucuronide ，isochlorogenic acid A ，apigenin-7-O-β-D- glucuronide，isochlorogenic acid C and daidzein . The results of cluster analysis and principle component analysis showed that 10 batches of Compound huiqin granules could be clustered into 4 categories，in which S 2，S3 and S 6 belonged to one category ，S4， S8，S10 belonged to one category ，S7 belonged to one category ，and S 1，S5，S9 belonged to one category . Average contents of 8 active components were 1.30，17.42，3.51，3.57，0.75，0.41，0.31，0.32 mg/g，respectively. CONCLUSIONS In this study ，the fingerprints and multi -component content determination method of Compound huiqin granules are successfully established ，which can provide a basis for the quality control of the granules .
