Analysis of essential oil from medicinal and non-medicinal parts of Atractylodes chinensis and antibacterial activity in vitro
- VernacularTitle:北苍术药用和非药用部位挥发油成分分析及体外抗菌活性考察
- Author:
Jianyun ZHANG
1
,
2
;
Yanyun ZHAO
1
,
2
;
Yunsheng ZHAO
1
,
2
;
Zongsheng YAN
1
,
2
;
Litao LU
1
;
Yuguang ZHENG
1
,
2
,
3
;
Huiyong FANG
1
Author Information
1. College of Pharmacy，Hebei University of Chinese Medicine/Hebei Province Processing Technology Innovation Center of Traditional Chinese Medicine，Shijiazhuang 050200，China
2. International Joint Research Center of Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province，Shijiazhuang 050200，China
3. Hebei Chemical and Pharmaceutical College，Shijiazhuang 050026，China
- Publication Type:Journal Article
- Keywords:
Atractylodes chinensis;
non-medicinal parts;
medicinal parts;
essential oil;
antibacterial activity;
component
- From:
China Pharmacy
2022;33(21):2609-2614
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the difference in chemical components of essential oil from medicinal part （rhizome）and non-medicinal parts （fibrous roots ，stems，and leaves ）of Atractylodes chinensis，and to preliminarily evaluate their antibacterial activity in vitro . METHODS The essential oil was extracted from three parts of A. chinensis by steam distillation ，and their chemical components and relative contents were analyzed and identified by gas chromatography -mass spectrometry （GC-MS）. The antibacterial activities of essential oil （5 mg/mL） from different parts against Staphylococcus aureus ，S. albus and Bacillus subtilis were determined by paper diffusion method . The correlation between chemical components in essential oil and antibacterial activity was analyzed . RESULTS A total of 60 chemical constituents were identified from the essential oilof rhizome ，fibrous roots ，stems and leaves of A. chinensis， mainly composed of terpenes ，sesquiterpenoids，alcohols and ketones. There were 8 kinds of common components in the essential oil of rhizome ，fibrous root and stem and leaves ，which were elementol ，cadinene，agarospirol，valencian citrine ， caryophyllin，β-sesquidiene，γ-eucalyptol and β-eucalyptol. A total of 26 components were identified from the essential oil of rhizome，of which the relative content of 4-benzaldehyde was the highest （31.56%）；a total of 21 components were identified from the essential oil of fibrous roots ，among which the relative content of β-eucalyptol was the highest （44.39%）；a total of 42 components were identified from the essential oil of stems and leaves ，among which the relative content of β-sesquidiene was the highest（14.83%）. The essential oil from different parts had certain antibacterial effect on S. aureus，S. albus and B. subtilis. The order of antibacterial effect was rhizome ＞fibrous roots ＞stem and leaves ；the relative contents of β-caryophyllene，α-humulene and modhephene were positively correlated with inhibitory effects of essential oil against S. aureus and B. subtilis（P＜0.05 or P＜0.01）. The relative contents of atractylone ，zingiberene and agarospirol were positively correlated with the inhibitory effect of essential oil against B. subtilis（P＜0.05 or P＜0.01）. CONCLUSIONS There are significant differences in the chemical composition and contents of essential oil from medicinal and non -medicinal parts of A. chinensis，and all of them have antibacterial activity in vitro .
