Chemical constituents of the n-butanol fraction from the stems of Clerodendrum trichotomum and their antitumor activities in vitro
- VernacularTitle:海州常山茎正丁醇部位的化学成分及体外抗肿瘤活性研究
- Author:
Linzhen LI
1
,
2
,
3
;
Yu ZHANG
1
,
2
,
3
;
Liang CHEN
1
,
2
,
3
;
Yinzhi CEN
1
,
2
,
3
;
Yangli TU
1
,
2
,
3
;
Xiaosheng YANG
2
;
Yongjun LI
3
Author Information
1. School of Pharmacy，Guizhou Medical University，Guiyang 550025，China
2. State Key Laboratory of Functions and Applications of Medicinal Plants，Guizhou Medical University，Guiyang 550004，China
3. Engineering Research Center for the Development and Application of Ethnic Medicine and TCM （Ministry of Education），Guizhou Medical University，Guiyang 550004，China
- Publication Type:Journal Article
- Keywords:
Clerodendrum trichotomum;
stem;
n-butanol fraction;
chemical constituents;
structural identification;
antitumor
- From:
China Pharmacy
2022;33(21):2578-2583
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To separate and identify the chemical constituents of the n-butanol fraction from the stems of Clerodendrum trichotomum ，and to investigate their antitumor activities in vitro . METHODS The ethanol extracts were obtained with 85% ethanol from dried stems of C. trichotomum. After dispersed with water ，ethanol extracts were distributed by petroleum ether，ethyl acetate and n-butanol in turn ，then concentrated under reduced pressure to obtain the fractions of each extraction part . The n-butanol fraction from the stems of C. trichotomum was isolated and purified by macroporous resin D 101 column chromatography and various chromatographic techniques including silica gel ，hydroxypropyl glucan gel and Toyopearl HW -40F macroporous resin and so on . The structures of them were identified by physical and chemical properties ，MS and NMR . All these compounds were evaluated for cytotoxic activities against 4 kinds of human tumor cells such as cultured K 562，MCF-7，A549 and HepG2，using the MTT assay . RESULTS Fourteen chemical constituents were isolated and identified as teuvincenone B （1）， uncinatone（2），villosin C （3），syringaresinol（4），syringaresinol-4ʹ-O-β-glucopyranoside（5），3，12-O-β-D-diglucopyranosyl-11，16- dihydroxyabieta-8，11，13-triene（6），glypentoside C （7），martynoside（8），isomartynoside（9），2-（4-hydroxyphenyl）ethanol-O-β-D- glucopyranosyl-（1→2）-O- β -D-glucopyranoside（10），3，4-dimethoxyphenyl-1-O- β -D-apiofuranosyl （1→2） - β -D- glucopyranoside（11）， 2，6-dimethoxy-4-hydroxy-1-O- β -D- glucopyranoside（12），adenosine（13）and cistanoside F （14）. In vitro anti-tumor activity studies showed that compounds 1-3 showed certa in inhibitory activities against tumor cellproliferation，among which compound 2 displayed the strongest inhibitory activity against MCF -7，A549 and HepG 2 cells，and their IC 50 values were 25.00，22.34 and 12.50 μmol/L respectively ；only compound 3 showed stronger inhibitory activity against K562 cell with IC 50 of 28.41 μmol/L. CONCLUSIONS Among them ，compounds 10 to 13 are isolated from genus Clerodendrum for the first time ，compounds 4，5，14 were isolated from C. trichotomum for the first time . The abietane diterpenoids （compounds 1-3）have better inhibitory activities against above four tumor cell lines .
