Preliminary study on metabolites derived from the ethanol extract from the leaves of Dimocarpus longan in rats in vivo
- VernacularTitle:龙眼叶乙醇提取物在大鼠体内的代谢产物初步研究
- Author:
Jue HU
1
,
2
;
Guangqiang HUANG
1
;
Jie LIANG
1
,
3
,
4
;
Xianfu LIU
5
;
Yupin CAO
1
,
5
;
Kuikui CHEN
1
;
Yaohua LI
1
,
2
;
Shijia AN
1
;
Jingchun LIANG
1
Author Information
1. School of Pharmacy，Guangxi University of Chinese Medicine，Nanning 530200，China
2. Key Laboratory of Chinese Medicine Extraction Purification and Quality Analysis of Guangxi Universities and Colleges，Nanning 530200，China
3. South China Branch of National Engineering Research Center for Manufacturing Technology of Traditional Chinese Medicine Solid Preparation，Nanning 530200，China
4. Guangxi Engineering and Technology Center for Zhuang Yao Medicine，Nanning 530200，China
5. Faculty of Chinese Medicine Science，Guangxi University of Chinese Medicine，Nanning 530222，China
- Publication Type:Journal Article
- Keywords:
Leaves of Dimocarpus longan;
ethanol extract;
UPLC-Q-TOF-MS/MS;
feces;
urine;
metabolites
- From:
China Pharmacy
2022;33(21):2572-2577
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the metabolites derived from the ethanol extract from the leaves of Dimocarpus longan preliminarily in rats in vivo ，and to provide reference for elucidating the possible metabolic mechanism of the leaves of D. longan in lowering blood glucose . METHODS Ultra high performance liquid chromatography quadrupole time -of-flight mass spectrometry （UPLC-Q-TOF-MS/MS） was adopted by taking ethanol extract of D. longan leaves，the feces and urine of rats at 0-72 h and 0-48 h after intragastric administration of 33.8 g/kg ethanol extract of D. longan leaves（by extract ），the feces and urine of rats at the corresponding time after intragastric administration of normal saline （blank control ） as samples . The accurate relative molecular weight ，formula and fragment information of the compounds were collected ， and the compounds were speculated and i dentified by matching with the database and spectrum library of the instrument ，and comparing with the reference substance and relevant literature . RESULTS A total of eight compounds were identified in urine and feces of rats ，including 2 prototype components and 6 metabolites. Three compounds （including two prototype components as quercetin ，luteolin and one metabolite as luteolin or kaempferol） in feces of rats were identified ；five compounds （all metabolites ） in urine of rats were identified ，involving metabolites of quercetin ，luteolin or kaempferol . Metabolites mainly included the products of methylation ，glucuronidation and oxidation. CONCLUSIONS After intragastric administration ，the ethanol extract from the leaves of D. longan is mainly metabolized in rats through methylation ，glucuronidation and other pathways . The identified compounds are mostly metabolites of quercetin and luteolin .
