Establishment of quantitative analysis method and prediction of potential mechanism for quality control components of Tenghuang jiangu capsules
- VernacularTitle:藤黄健骨胶囊质控成分定量分析方法建立及潜在作用机制预测
- Author:
Lin ZHOU
1
,
2
;
Xiaohui WANG
3
;
Zhi SUN
1
;
Lianping XUE
1
;
Jianwen JIN
4
;
Jing WU
4
;
Xiaojing LI
1
;
Tianyuan ZHENG
1
;
Xiaojian ZHANG
1
Author Information
1. Dept. of Pharmacy，the First Affiliated Hospital of Zhengzhou University，Zhengzhou 450052，China
2. School of Minerals Processing and Bioengineering，Central South University，Changsha 410000，China
3. Dept. of Ultrasound，the First Affiliated Hospital of Zhengzhou University，Zhengzhou 450052，China
4. Shaanxi Licai Pharmaceutical Co.，Ltd.，Shaanxi Xianyang 712000，China
- Publication Type:Journal Article
- Keywords:
Tenghuang jiangu capsule;
quality control
- From:
China Pharmacy
2022;33(22):2743-2747
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a quantitative analysis method for the quality control components in Tenghuang jiangu capsules， and predict the possible action mechanism of the quality control components. METHODS Seven key quality control components in Tenghuang jiangu capsules were quantitatively analyzed by UHPLC-Q-Orbitrap HRMS. The “component-target” network was constructed based on network pharmacology， and the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment analysis and gene ontology （GO） function enrichment analysis were further conducted to find the key signaling pathways. RESULTS The average contents of succinic acid， hyperoside， gallic acid， kaempferol， naringin， naringenin and protocatechuic acid in 20 batches of Tenghuang jiangu capsules were 520.92， 67.67， 129.48， 4.74， 397.45， 5.66 and 376.62 μg/g， respectively. The results of network pharmacology showed that the 62 key target genes of the quality control components of the drug included AKT1， TNF， VEGFA， MMP9， PTGS2， etc. They were mainly enriched in cytokine receptor interaction， nuclear factor， tumor necrosis factor， interleukin 17， rheumatoid arthritis， Toll-like receptor and other signal pathways， involving inflammatory reaction， signal transduction， protein phosphorylation and other biological processes， kytoplasm， cell membrane and other cell components， as well as enzyme activity， energy activity and other molecular functions. CONCLUSIONS The established UHPLC- Q-Orbitrap HRMS method can be used for the quantitative analysis of the quality control components of Tenghuang jiangu capsule. Its quality control components may be mapped to inflammatory pathways related to bone diseases such as rheumatoid arthritis and Toll-like receptors through AKT1， TNF， VEGFA and other key targets， so as to play a therapeutic role.
