Optimization of the extraction and separation process of chebulagic acid and chebulinic acid
- VernacularTitle:诃黎勒酸和诃子酸的提取、分离工艺优化
- Author:
Jiumei CHEN
1
;
Qizhen ZHU
1
;
Chengguo JU
1
;
Qiang ZHANG
1
;
Wei WANG
1
Author Information
1. School of Pharmacy，Liaoning University of Traditional Chinese Medicine，Liaoning Dalian 116600，China
- Publication Type:Journal Article
- Keywords:
chebulagic acid;
chebulinic acid;
extraction process;
separation process
- From:
China Pharmacy
2022;33(22):2733-2737
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize the extraction and separation process of chebulagic acid and chebulinic acid from Terminalia chebula. METHODS Based on single factor experiment， with particle size， liquid-solid ratio， extraction time and extraction times as factors， using the contents of chebulagic acid and chebulinic acid as indexes， orthogonal experiment was designed to optimize the extraction process of chebulagic acid and chebulinic acid. Taking sample concentration， elution solvent and the ratio of eighteen-group bonded silicone reverse phase （ODS） to the amount of raw medicine as factors， the separation processes of chebulagic acid and chebulinic acid were optimized. RESULTS The optimal extraction process of chebulagic acid and chebulinic acid included ethanol volume fraction of 70%， ultrasonic extraction， particle size of 120 mesh， liquid-solid ratio of 25∶1 （mL/g）， extraction time of 20 min， and extracting for 2 times. After 3 experiments， the average comprehensive score was 99.33 （RSD＝ 0.68%， n＝3）， and the average contents of chebulagic acid and chebulinic acid were 107.05 and 58.32 mg/g， respectively. The optimal separation process of the two components included the concentration of sample loading solution was 0.5 g/mL （1 mL was equivalent to 0.5 g of medicinal materials）， the ratio of ODS to the amount of raw medicine was 10∶1.5 （g/g）， methanol-water （1∶4， V/V） eluted chebulagic acid， methanol-water （3∶7， V/V） eluted chebulinic acid. After 3 experiments， the average total yields of the two components were 53.33%， 39.23%. After recrystallization， the purity of both components was 100%. CONCLUSIONS Established extraction and separation process of chebulagic acid and chebulinic acid is simple and feasible.
