Prevention of renal interstitial fibrosis by osthole in diabetic nephropathy model mice
- VernacularTitle:蛇床子素对糖尿病肾病模型小鼠肾间质纤维化的预防作用
- Author:
Qian HUANG
1
;
Dandan ZHENG
1
;
Qiuhong HUANG
1
;
Zilu SHI
2
Author Information
1. Teaching and Research Section of Physiology，Basic Medicine Department，Quanzhou Medical College，Fujian Quanzhou 362100，China
2. Dept. of Nephrology，Quanzhou First Hospital Affiliated to Fujian Medical University，Fujian Quanzhou 362000，China
- Publication Type:Journal Article
- Keywords:
osthole;
diabetic nephropathy;
interstitial fibrosis
- From:
China Pharmacy
2022;33(22):2719-2723
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of osthole （OST） on renal interstitial fibrosis in diabetic nephropathy （DN） model mice. METHODS The diabetic mice model was established by the tail vein injection of streptozotocin once， and the DN model was established by feeding for 12 weeks after successful modeling of diabetes. Diabetic model mice were randomly divided into model group， low-dose， moderate-dose， high-dose groups （20， 40， 80 mg/kg） of OST， with 10 mice in each group. After successful modeling of diabetes， the mice were given corresponding drugs or solvent （model group） intragastrically， once a day， for consecutive 12 weeks， and the normal control group was set up at the same time. After the last administration， the levels of fasting blood glucose， 24 h urinary protein， serum creatinine （Scr） and blood urea nitrogen were tested in each group. Masson staining was used to observe the deposition of collagen fibers in renal interstitium； the expressions of E-cadherin and vimentin in renal cortex were detected by immunohistochemical staining. The protein expressions of follistatin-like protein 1 （Fstl1） and Snail family transcriptional repressor 1 （Snail1）， the phosphorylation of protein kinase B （Akt） （calculated by p-Akt/Akt） in the renal cortex were detected by Western blot. RESULTS Compared with normal control group， the levels of fasting blood glucose， 24 h urinary protein， Scr and blood urea nitrogen， collagen fiber deposition ratio of renal interstitium， the expression of vimentin， protein expressions of Fstl1 and Snail1， p-Akt/Akt in renal cortex were increased significantly （P＜0.01）， as well as the expression of E-cadherin was decreased significantly （P＜0.01）. Compared with model group， above indexes of OST groups were reversed significantly （P＜0.05 or P＜0.01）. CONCLUSIONS Preventive use of OST can effectively reduce fasting blood glucose level， protect renal function and inhibit epithelial-mesenchymal transition of DN model mice， and delay the progression of renal interstitial fibrosis ， the mechanism of which may be associated with the suppression of Fstl1/Akt/Snail1 signaling pathway.
