Study on the “property-effect”correlation material basis of Taohe chengqi decoction in removing blood stasis and purging heat
- VernacularTitle:桃核承气汤化瘀泻热“性-效”相关物质基础研究
- Author:
Wanfen SHU
1
;
Wen LIU
2
;
Xinli SONG
1
;
Xingde LIU
1
;
Ganchun ZHANG
1
;
Qin QIN
1
;
Hongxin WANG
1
Author Information
1. School of Pharmacy，Guizhou University of Traditional Chinses Medicine，Guiyang 550025，China
2. School of Pharmacy，Guizhou Medical University，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
Taohe chengqi decoction;
blood storage syndrome;
medical property;
coagulation index;
inflammatory indicator
- From:
China Pharmacy
2022;33(22):2706-2711
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the “property-effect” correlation material basis of the classic famous prescription Taohe chengqi decoction in removing blood stasis and purging heat. METHODS The rat model of blood storage syndrome was established to investigate the effects of Taohe chengqi decoction （17.29 g/kg） and its property grouping components [bitter-cold property group （Rhei Radix et Rhizoma+Natrii Sulfas） 9.43 g/kg， pungent-warm property group （Cinnamomi Ramulus+Persicae Semen） 4.71 g/kg， sweet-flat property group （Glycyrrhizae Radix et Rhizoma） 3.14 g/kg] on coagulation indexes （prothrombin time， prothrombin activity， activated partial thrombin time， fibrinogen， thrombin time and endothelin-1） and inflammation indicators （C- reactive protein， tumor necrosis factor-α and superoxide dismutase） in rats. On the basis of determining the dominant property groups， ultra-performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry was used to identify the components. The molecular docking was performed， and the components with binding energy ≤－5.0 kcal/mol were selected as the basis of “property-effect” correlation material basis. RESULTS Taohe chengqi decoction total prescription group and pungent-warm property group could significantly improve the coagulation indexes of model rats （P＜0.05 or P＜0.01）， and the total prescription group and bitter-cold property group could significantly improve the inflammation indexes of model rats （P＜0.05 or P＜0.01）. The pungent-warm property group was the dominant property group for removing blood stasis，and the bitter-cold property group was the dominant property group for purging heat. Thirty-two and thirty-five components were identified from the pungent-warm property group and bitter-cold property group， respectively. With binding energy ≤－5.0 kcal/mol， there were 10 components （abscisic acid， 3，4-dihydroxypheny- lethanol， procyanidin B1， etc.） in the pungent-warm property group and 13 components （baicalein， aloe-emodin， demethylwedelolactone， etc.） in the bitter-cold property group. CONCLUSIONS Taohe chengqi decoction has the effect of removing blood stasis and purging heat， and its material basis comes from pungent-warm property group （Cinnamomi Ramulus， Persicae Semen） and bitter-cold property group （Rhei Radix et Rhizoma， Natrii Sulfas） respectively.
