Interleukin-9 promotes the activation of hepatic stellate cells in mice infected with Schistosoma japonicum
10.16250/j.32.1374.2021282
- VernacularTitle:白细胞介素9促进日本血吸虫感染小鼠 肝星状细胞激活
- Author:
Ting-zheng ZHAN
1
;
Hui-hui MA
2
;
Qing LI
3
;
Li-li TANG
1
;
Shan-shan HE
1
;
Ze-li TANG
3
;
Chao-ming XIA
2
Author Information
1. Department of Parasitology, Guangxi Medical University, Nanning, Guangxi 530021, China
2. Department of Pathogenic Biology, Medical College, Soochow University, Suzhou, Jiangsu 215000, China
3. Department of Cell Biology, Guangxi Medical University, China
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Interleukin-9;
Hepatic stellate cell;
Hepatic fibrosis
- From:
Chinese Journal of Schistosomiasis Control
2022;34(5):514-517
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of interleukin-9 (IL-9) in the activation of hepatic stellate cells (HSCs) in mice infected with Schistosoma japonicum. Methods Primary HSCs were isolated from mice 7 weeks post-infection with S. japonicum using the in situ liver perfusion and density gradient centrifugation, and cultured in vitro. HSCs were randomly assigned to the PBS control group and IL-9 stimulation group (stimulation with 20 ng/mL IL-9). HSCs were harvested 48 h and 72 h poststimulation, and the expression of α-smooth muscle actin (α-SMA), type I collagen (Col I) and type III collagen (Col III) was determined in HSCs using Western blotting. Results Following stimulation with 20 ng/mL IL-9 for 48 h, the expression of α-SMA [(0.87 ± 0.02) vs. (0.69 ± 0.01); t = 17.39, P < 0.01], Col I [(0.74 ± 0.02) vs. (0.65 ± 0.01); t = 9.56, P < 0.01] and Col III [(0.94 ±0.04) vs. (0.75 ± 0.03); t = 6.15, P < 0.01] was significantly greater in HSCs in the IL-9 stimulation group than in the PBS control group. Following stimulation with 20 ng/mL IL-9 for 72 h, the expression of α-SMA was significantly greater in HSCs in the IL-9 stimulation group than in the PBS control group[(0.76 ± 0.02) vs. (0.58 ± 0.02); t = 12.52, P < 0.01]; however, there were no significant differences between the two groups in terms of Col I [(0.68 ± 0.02) vs. (0.66 ± 0.02); t = 1.15, P > 0.05] or Col III expression [(0.75 ± 0.01) vs. (0.72 ± 0.02); t = 2.22, P > 0.05]. Conclusions IL-9 promotes the activation of HSCs in mice infected with S. japonicum.
