Establishment and evaluation of pathologic model of CD1 mice infected with Streptococcus suis serotype 7
10.3969/j.issn.1006-2483.2022.06.016
- VernacularTitle:猪链球菌7型CD1小鼠动物病理模型的建立及评价
- Author:
Xiao-yan TANG
1
;
Die LIN
2
;
Ling PENG
2
Author Information
1. Medical College, Shaoguan University, Shaoguan, Guangdong 512000 , China
2. Henry Fok College of Biology and Agriculture, Shaoguan University , Shaoguan , Guangdong 512000 , China
- Publication Type:Journal Article
- Keywords:
Streptococcus suis type 7;
CD1 mice;
Model;
Evaluation
- From:
Journal of Public Health and Preventive Medicine
2022;33(6):68-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the pathogenicity of Streptococcus suis type 7 in CD1 mice and to explore the feasibility of CD1 mice as an animal infection model of Streptococcus suis type 7. Methods CD1 mice were infected with Streptococcus suis type 7 isolated from Guangdong Province by intraperitoneal inoculation. The pathological model of CD1 mice was evaluated by the observation of clinical symptoms, pathological autopsy, histopathological examination of liver and brain tissue sections, determination of LD50, and identification of bacteria and detection of bacterial load in tissues and organs. Results The pathological incidence of infected CD1 mice showed good regularity and repeatability. The clinical symptoms, pathological changes and tissue damage of infected CD1 mice were basically consistent with the previous experimental results of Streptococcus suis type 2 and 9 infection in mice and were similar to the natural disease of pigs. Live bacteria could be isolated from blood, heart, liver, spleen, lung, kidney and brain of infected mice, and the recovered bacteria were consistent with the original bacteria. The detection of bacterial load in tissues and organs showed that the bacterial load in heart was the highest, followed by liver, kidney, spleen, lung and blood, and the bacterial load in brain was the lowest. Conclusion In this study, we demonstrate that Streptococcus suis type 7 strain can cause sepsis and brain inflammation in CD1 mice, resulting in tissue damage and death. CD1 mice are sensitive to the experimental strain and can be used as an animal model of streptococcus suis type 7 infection.
