Baicalin alleviates cortical neuronal cell injury in rats with cerebral ischemia-reperfusion through modulating inflammatory factors
10.3969/j.issn.1006-2483.2022.06.007
- VernacularTitle:黄芩苷调控炎症因子缓解脑缺血再灌注大鼠皮层神经细胞损伤的研究
- Author:
Bi-hua LYU
1
;
Chuan-zhen XIONG
2
;
Yang ZHANG
2
;
Lei CHEN
2
Author Information
1. Department of Pharmacy , Zhongnan Hospital, Wuhan University , Wuhan , Hubei 430071 , China
2. Department of Environmental Health and Occupational Medicine , School of Public Health, Wuhan University of Science and Technology , Wuhan , Hubei 430065 , China
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia-reperfusion;
Baicalin;
G protein-coupled receptor 30（GPR30）;
Toll-like receptor 4（TLR4）;
NACHT-LRR-PYD-containing proteins 3（NLRP3）
- From:
Journal of Public Health and Preventive Medicine
2022;33(6):28-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of baicalin on cortical nerve cell injury in rats with cerebral ischemia-reperfusion. Methods Twenty-eight SD rats were divided into four groups according to a random number table: sham-operated group, model group, baicalin low concentration (100 mg/kg) group, baicalin high concentration (200 mg/kg) group, 7 rats in each group. Cerebral ischemia was constructed by middle cerebral artery embolization method with 2h cerebral ischemia followed by reperfusion for 24 h. Subsequently, neurological function score was performed by Longa's 5-point method; 2,3,5-triphenyltetrazolium chloride (TTC) staining was used to evaluate the volume of cerebral infarction; HE staining was used to observe cortical neuronal cell damage; immunofluorescence staining was used to detect the expression of inflammation-related factors GPR30, TLR4 and NALP3 . Results The neurological function scores of the four groups of rats were 0, 3.2±0.3, 2.6±0.2, and 2.2±0.18, respectively, and the percentages of cerebral infarct volume were 0, (33.84±2.12)%, (26.53±1.89)%, and (20.45±1.32)%, respectively. HE staining results showed that the neuronal cells in the cortical area of the model group underwent significant necrosis compared with the sham-operated group, and while the necrosis of cortical nerve cells was significantly improved by the treatment with baicalin. Immunofluorescence results showed that compared with the sham-operated group, the fluorescence intensity of TLR4 and NALP3 proteins was enhanced and the fluorescence intensity of GPR30 was reduced in the model group, while the fluorescence intensity of TLR4 and NALP3 proteins was reduced and GPR30 fluorescence intensity was enhanced after baicalin treatment. Conclusion Cerebral Ischemia-reperfusion injury leads to cortical damage and mediates the inflammatory response of neuronal cells in rats, and baicalin could alleviate the neurological damage caused by cerebral ischemia-reperfusion by reducing the inflammatory response.
