Effects of hydroquinone on autophagy and Akt/Foxo3a signal pathway in Jurkat cells
10.3969/j.issn.1006-2483.2022.06.004
- VernacularTitle:氢醌对Jurkat细胞自噬及Akt/Foxo3a信号通路的影响
- Author:
Huan LIU
1
;
Hai-hong JIANG
1
;
Wei CAI
1
;
Yong-yi BI
1
;
Yi-ni LIU
1
;
Ge LIU
1
;
Hong WANG
1
Author Information
1. School of Public Health , Wuhan University , Wuhan , Hubei 430071 , China
- Publication Type:Journal Article
- Keywords:
Hydroquinone;
Jurkat cells;
Autophagy;
Bioinformatic analysis;
Akt/Foxo3a signal pathway
- From:
Journal of Public Health and Preventive Medicine
2022;33(6):15-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of HQ on autophagy in human leukemia Jurkat T-cells and its mechanism. Methods (1) Exponentially growing Jurkat cells were cultured in vitro with HQ at the concentrations of 0 (control group), 12.5 and 25 mol·L-1 for 24 hours. Western blotting was used to detect the protein levels of autophagy markers P62 and LC3II. Aggregations of LC3 fluorescent spots were determined by immunofluorescence technique. (2) Using the gene expression database, differentially expressed microRNAs (miRNAs) in CD34+ cells treated with HQ were selected to predict target genes through gene prediction tools such as TargetScan, miRBD and miRwalk. Enrichment analysis was conducted on these target genes to obtain relevant signal pathways. (3) Based on the results above, RT-PCR was used to examine the expression of Akt and Foxo3a genes in Akt/Foxo3a signal pathway, while western blotting was performed to determine protein expression levels of Akt, p-Akt, Foxo3a and p-Foxo3a in Jurkat cells exposed to HQ. Results (1) Compared with the control group, the expression of autophagy key protein P62 decreased in 25mol·L-1 group, and the ratio of LC3II/LC3I increased in 12.5mol·L-1 and 25mol·L-1 groups (P<0.05). The results of immunofluorescence showed that the aggregation of LC3 fluorescent spots increased with the increase of HQ concentration. (2) Bioinformatic analysis revealed several autophagy-related signaling pathways including AMPK, MAPK, Hedgehog and PI3K/Akt signal pathways. (3) HQ treatment decreased gene expression levels of Akt and Foxo3a in Akt/Foxo3a signal pathway, and the protein levels of Akt, p-Akt, Foxo3a and p-Foxo3a were also significantly decreased in cells treated with HQ in a concentration dependent manner (P<0.05). Conclusion HQ may promote autophagy in Jurkat cells, which may be partly mediated through the inhibition of Akt/Foxo3a signal pathway..
