Chemical Consistency of Lycii Cortex from Different Origins Based on Component Contents and Fingerprint
10.13422/j.cnki.syfjx.20220461
- VernacularTitle:基于含量测定和指纹图谱的不同基原地骨皮药材异同点考察
- Author:
Baoxin LI
1
;
Lixia HAO
1
;
Yuntao DAI
1
;
Xiwen LI
1
;
Lan WU
1
Author Information
1. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijng 100700，China
- Publication Type:Journal Article
- Keywords:
Lycii Cortex;
medicinal material origin;
ultra performance liquid chromatography （UPLC）;
fingerprint;
kukoamine B;
chemical consistency evaluation;
famous classical formulas
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(24):179-185
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveIn this study， the two different origins of Lycii Cortex in the 2020 edition of Chinese Pharmacopoeia were determined to analyze their chemical consistency by comparing their main chemical composition. MethodThirty representative batches of Lycii Cortex were collected， content determination and fingerprint analysis methods were established by ultra performance liquid chromatography-photodiode array detector （UPLC-PDA） combining with multivariate statistical analysis to evaluate the similarities and differences between two origins of Lycii Cortex. Respectively by the mobile phase of acetonitrile （A）-0.15% trifluoroacetic acid aqueous solution （B） and the mobile phase of acetonitrile （A）-0.1% formic acid aqueous solution （B） for gradient elution （0-4 min， 5%-12%A； 4-8 min， 12%A； 8-12 min， 12%-14%A； 12-15 min， 14%-30%A； 15-17 min， 30%-40%A； 17-18 min， 40%-90%A）， and the detection wavelength was set at 280 nm. ResultThis established content determination and fingerprint methods had good precision， stability and repeatability. The similarities of 30 batches of Lycii Cortex were above 0.90 by comparing with the control fingerprint， and the eight common peaks in fingerprints of Lycii Cortex from Lycium barbarum and L. chinense were all phenolic amides， which were kukoamine B， N-（4，9，13-triazatridecan-1-yl）-3，4-dihydroxybenzenepropanamide， feruloylputrescine， N1，N5-bis （dihydrocaffeoyl） spermidine or N5，N10-bis （dihydrocaffeoyl） spermidine， N5-caffeoyl-N10-dihydrocaffeoylspermidine， N5-dihydrocaffeoyl-N10-caffeoylspermidine， N1，N5-bis （caffeoyl） spermidine and lyciumin A. Among them， the content ranges of kukoamine B in Lycii Cortex from L. chinense and L. barbarum were 1.22%-8.18%， 2.52%-12.24%， respectively. ConclusionThe established UPLC analysis method can be used for the content determination and fingerprint analysis of Lycii Cortex. This study indicates that chemical contour of Lycii Cortex from L. barbarum and L. chinense are similar， there are no significant differences in kukoamine B content， and they have consistency in the chemical composition.
