Metabolic Analysis of Tongfengding Capsules in vivo Based on Sequential Metabolism
10.13422/j.cnki.syfjx.20220853
- VernacularTitle:基于序贯代谢方法的痛风定胶囊体内代谢分析
- Author:
Hongjiao CHEN
1
;
Wenning YANG
1
;
Renfang YIN
2
;
Yan ZHU
2
;
Dongying QI
1
;
Xueyan LI
1
;
Huining LIU
1
;
Shuang YU
1
;
Yanli PAN
2
Author Information
1. School of Chinese Materia Medica，Beijing University of Chinese Medicine，Beijing 102488，China
2. Institute of Information on Traditional Chinese Medicine，China Academy of Chinese Medical Sciences，Beijing 100700，China
- Publication Type:Journal Article
- Keywords:
Tongfengding capsules;
sequential metabolism;
ultra-high performance liquid chromatography-high resolution mass spectrometry （UPLC-HRMS）;
hydroxylation;
glucuronidation;
alkaloids;
terpenoids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(24):160-170
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveBased on sequential metabolism and ultra-high performance liquid chromatography-high resolution mass spectrometry （UPLC-HRMS）， to identify the prototype components and metabolites of Tongfengding capsules in rat plasma. MethodAn ACQUITY UPLC BEH Shield RP18 column （2.1 mm×100 mm， 1.7 µm） was used for gradient elution with 0.1% formic acid aqueous solution （A）-acetonitrile （B） as the mobile phase （0-1 min， 5%B； 1-2.4 min， 5%-10%B； 2.4-13.5 min， 10%-32%B； 13.5-18.5 min， 32%-90%B； 18.5-19 min， 90%-5%B； 19-21 min， 5%B） at a flow rate of 0.3 mL·min-1， injection volume of 2 μL and column temperature at 35 ℃. The heated electrospray ionization （HESI） was applied under positive and negative ion modes， and the scanning range was m/z 100-1 500. By comparing the differences between the administered plasma and the blank plasma， the prototype components and metabolites in intestinal metabolism sample and liver metabolism sample prepared by intestinal perfusion with parallel blood collection， and comprehensive metabolism sample prepared by intragastric administration method were identified. ResultA total of 76， 53， 74 chemical components were detected in the intestinal metabolism sample， the liver metabolism sample and the comprehensive metabolism sample. A total of 100 components were identified from these different plasma samples， including 64 prototype components （34 alkaloids， 12 terpenoids， 9 organic acids， 6 flavonoids and 3 other components） and 36 metabolites. The main metabolic reactions involved in the formation of metabolites were glucuronidation， deglucosylation， dehydrogenation and hydroxylation. ConclusionThe chemical components of Tongfengding capsules can undergo a series of metabolic reactions in the intestine and liver， and a large number of metabolites are generated， among which alkaloids may be the leading component group for efficacy， which can lay the foundation for systematic elucidating the material basis of Tongfengding capsules in vivo.
