Effect of Berberine Combined with Evodiamine on Migration and Invasion of Colorectal Cancer Cells
10.13422/j.cnki.syfjx.20221529
- VernacularTitle:小檗碱与吴茱萸碱联用对结直肠癌细胞迁移、侵袭能力的影响
- Author:
Ningning CHEN
1
;
Yifang JIANG
1
;
Yi YANG
1
;
Ziyi ZHAO
1
;
Fengming YOU
1
;
Ran YAN
1
Author Information
1. Traditional Chinese Medicine （TCM） Regulating Metabolic Diseases Key Laboratory of Sichuan Province，Hospital of Chengdu University of TCM，Chengdu 610075，China
- Publication Type:Journal Article
- Keywords:
berberine;
evodiamine;
colon cancer;
cell migration;
cell invasion;
phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(24):98-104
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of berberine combined with evodiamine on the migration and invasion of colorectal cancer HCT116 and RKO cells and to explore the underlying mechanism. Methodcell counting kit-8 （CCK-8） assay was used to examine the proliferation of HCT116 and RKO cells treated by berberine （30 μmol·L-1）， evodiamine （0.8 μmol·L-1）， and combination of two （30 μmol·L-1+0.8 μmol·L-1）， respectively. Scratch assay and Transwell assay were employed to detect the migration and invasion of HCT116 and RKO cells treated with berberine， evodiamine， and the combination， separately. In addition， the protein expression of epithelial cadherin （E-cadherin）， neural cadherin （N-cadherin）， phosphatidylinositol 3-kinase （PI3K）， and protein kinase B （Akt） in HCT116 and RKO cells treated with the berberine， evodiamine， and the combination was respectively measured by Western blot. ResultCompared with the blank group， berberine alone and evodiamine alone had no significant inhibitory effect on the proliferation， migration， and invasion of HCT116 and RKO cells， while the combination showed significant inhibition （P<0.01）. Berberine alone and evodiamine alone had no remarkable influence on the expression of PI3K， N-cadherin， and E-cadherin in HCT116 and RKO cells， but the combination significantly reduced the expression of PI3K and N-cadherin （P<0.01） and increased the expression of E-cadherin （P<0.01） in HCT116 and RKO cells. Evodiamine alone also significantly suppressed the expression of Akt protein in HCT116 and RKO cells （P<0.05）， but the suppression was weaker than that of the combination. ConclusionThe combination of berberine and evodiamine can significantly inhibit the migration and invasion of colorectal cancer HCT116 and RKO cells and the two show synergy. The mechanism is the likelihood that the combination down-regulates the expression of PI3K and Akt.
